Human-induced pluripotent stem cell (hiPSC)-derived cardiac patches have been extensively used for treating myocardial infarction and have shown potential for clinical application. However, the limited patch thickness can hamper its therapeutic effect. We previously developed a fibrous scaffold that allowed the formation of well-organized cardiac tissue constructs. In the present study, based on the above technology, we developed a three-dimensional multilayer fibrous scaffold with dynamic perfusion, on which approximately 20 million hiPSC-derived cardiomyocytes (CMs) could be seeded in a single step and organized into 1 mm thick and viable tissue. The multilayer cardiac tissue demonstrated enhanced contractile properties and upregulated cytokine secretion compared with the control group. Notably, when used on the myocardial infarction model, the multilayer group showed improved functional recovery and less fibrosis. These results indicated that the appropriate hiPSC-CM dose requires careful evaluation in developing clinical therapy. The multilayer cardiac tissue group demonstrated significant improvement than the control group, indicating that higher doses of transplanted cells may have improved therapeutic effects in treating myocardial infarction.

人诱导多能干细胞（hiPSC）来源的心脏补片已广泛用于治疗心肌梗塞，并显示出临床应用的潜力。然而，有限的贴片厚度会妨碍其治疗效果。我们之前开发了一种纤维支架，可以形成组织良好的心脏组织结构。在本研究中，基于上述技术，我们开发了一种具有动态灌注功能的三维多层纤维支架，可以在其上一步接种约2000万个hiPSC衍生的心肌细胞（CM）并组织成1毫米厚的组织。活组织。与对照组相比，多层心脏组织表现出增强的收缩特性和上调的细胞因子分泌。值得注意的是，当用于心肌梗死模型时，多层组显示出功能恢复改善和纤维化减少。这些结果表明，在开发临床治疗时需要仔细评估适当的 hiPSC-CM 剂量。多层心脏组织组比对照组表现出显着改善，表明更高剂量的移植细胞可能改善心肌梗死的治疗效果。