A mixture of six lignocellulosic biomasses could be a solution for sustainable biorefinery supply throughout the year compared with seasonal feedstock. In this study, a sequential process of autohydrolysis and organosolv was used for this mixture. To optimize lignin extraction, an experimental design was used to study the impacts of temperature, ethanol concentration, and time on lignin yield and related characteristics of the organosolv process. A highly pure organosolv lignin (OL) (ca. 90% Klason lignin) was recovered. FTIR analysis showed the main lignin structure was almost unchanged. NMR confirmed this and revealed the presence of guaiacyl-syringyl type with small amounts of p-hydroxyphenyl units (25% G, 70% S, and 5% H), predominantly presenting β-O-4 units (ca. 80%). Tg from 73 to 85 °C was detected. OL showed radical scavenging activity as high as the commercial antioxidant BHT. Moreover, enzymatic susceptibility of delignified biomass was accessed, in which 50% glucose yield was achieved.

与季节性原料相比，六种木质纤维素生物质的混合物可能是全年可持续生物炼制供应的解决方案。在本研究中，对该混合物使用了自水解和有机溶剂的连续过程。为了优化木质素提取，采用实验设计来研究温度、乙醇浓度和时间对木质素产量和有机溶剂工艺的相关特性的影响。回收了高纯度的有机溶剂木质素 (OL)（约 90% Klason 木质素）。FTIR分析显示主要木质素结构几乎没有变化。NMR 证实了这一点，并揭示了愈创木基-紫丁香基类型的存在，其中含有少量对羟基苯基单元（25% G、70% S 和 5% H），主要存在 β-O-4 单元（约 80%）。检测到 Tg 为 73 至 85 °C。OL 的自由基清除活性与商业抗氧化剂 BHT 一样高。此外，还获得了脱木质素生物质的酶敏感性，其中葡萄糖产率达到了 50%。