Planta ( IF 3.6 ) Pub Date : 2023-07-29 , DOI: 10.1007/s00425-023-04212-1 Xinhua Zhang 1 , Xiaohong Chen 1 , Jaime A Teixeira da Silva 2 , Ting Zhang 1 , Yuping Xiong 1 , Yuan Li 1 , Yunfei Yuan 1 , Xiaoping Pan 1 , Guohua Ma 1
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Main conclusion
Santalum album (E,E)-α-farnesene synthase catalyzes FPP into (E,E)-α-farnesene. Overexpression of the SaAFS gene positively improved cold stress tolerance through JA biosynthesis and signaling pathways in Arabidopsis.
Abstract
Volatile terpenoids are released from plants that suffer negative effects following exposure to various biotic and abiotic stresses. Recent studies revealed that (E,E)-α-farnesene synthase (AFS) plays a significant role in a plant’s defence against biotic attack. However, little is known about whether AFS contributes to plant resistance to cold stress. In this study, a SaAFS gene was isolated from Indian sandalwood (Santalum album L.) and functionally characterized. The SaAFS protein mainly converts farnesyl diphosphate to (E,E)-α-farnesene. SaAFS was clustered into the AFS clade from angiosperms, suggesting a highly conserved enzyme. SaAFS displayed a significant response to cold stress and methyl jasmonate. SaAFS overexpression (OE) in Arabidopsis enhanced cold tolerance by increasing proline content, reducing malondialdehyde content, electrolyte leakage, and accumulating reactive oxygen species. Transcriptomic analysis revealed that upregulated genes related to stress response and JA biosynthesis and signaling were detected in SaAFS-OE lines compared with wild type plants that were exposed to cold stress. Endogenous JA and jasmonoyl-isoleucine content increased significantly in SaAFS-OE lines exposed to cold stress. Collectively considered, these results suggest that the SaAFS gene is a positive regulator during cold stress tolerance via JA biosynthesis and signaling pathways.
中文翻译：
檀香 (E,E)-α-法呢烯合酶的表征，其过度表达通过拟南芥中的茉莉酸生物合成和信号传导增强耐寒性
主要结论
檀香( E,E )-α-法呢烯合酶催化 FPP 生成 ( E,E )-α-法呢烯。SaAFS基因的过度表达通过 JA 生物合成和信号通路在拟南芥中积极提高了冷应激耐受性。
抽象的
暴露于各种生物和非生物胁迫后遭受负面影响的植物会释放挥发性萜类化合物。最近的研究表明，( E,E )-α-法呢烯合酶 (AFS) 在植物防御生物攻击方面发挥着重要作用。然而，关于 AFS 是否有助于植物抵抗冷胁迫，人们知之甚少。在本研究中，从印度檀香 ( Santalum album L.)中分离出SaAFS基因并进行功能表征。SaAFS蛋白主要将二磷酸法呢基转化为( E,E )-α-法呢烯。SaAFS 聚集到被子植物的 AFS 进化枝中，表明这是一种高度保守的酶。SaAFS对冷应激和茉莉酸甲酯表现出显着的反应。拟南芥中SaAFS过表达（OE）通过增加脯氨酸含量、减少丙二醛含量、电解质渗漏和积聚活性氧来增强耐冷性。转录组分析表明，与暴露于冷胁迫的野生型植物相比，在SaAFS -OE 品系中检测到与胁迫反应和 JA 生物合成和信号传导相关的上调基因。暴露于冷胁迫的SaAFS -OE 品系中内源 JA 和茉莉酰异亮氨酸含量显着增加。综合考虑，这些结果表明SaAFS基因通过 JA 生物合成和信号通路在冷应激耐受过程中发挥正向调节作用。
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