<br>用于软骨组织工程的两性离子颗粒水凝胶,Advanced Healthcare Materials

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用于软骨组织工程的两性离子颗粒水凝胶
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2023-07-27 , DOI: 10.1002/adhm.202301831
Maryam Asadikorayem ₁ , František Surman ₁ , Patrick Weber ₁ , Daniel Weber ₂ , Marcy Zenobi-Wong ₁

Affiliation

两性离子水凝胶具有超亲水性、非免疫原性和卓越的防污性能，因此在软骨组织工程中具有很高的潜力。然而，由于缺乏允许以生物相容性方式封装细胞的可注射两性离子水凝胶，到目前为止，它们在该领域的应用受到限制。在此，开发了一种新的策略来利用两性离子颗粒水凝胶来设计软骨，这些水凝胶是可注射的、自愈的、原位可交联的，并允许直接封装具有生物相容性的细胞。颗粒水凝胶是由两性离子羧基甜菜碱丙烯酰胺（CBAA）或 CBAA 和两性离子磺基甜菜碱甲基丙烯酸酯（SBMA）的混合物制成的块状光交联水凝胶的机械碎裂产生的。产生的微凝胶使用辣根过氧化物酶进行酶促交联，以快速稳定构建体，从而产生微孔水凝胶。包封的人原代软骨细胞具有高度活力，能够在两性离子颗粒水凝胶中增殖、迁移和产生软骨细胞外基质（ECM）。研究还表明，通过增加水凝胶孔隙率和 SBMA 的掺入，细胞增殖和 ECM 分泌得到进一步改善。该策略是一种简单且可扩展的方法，在扩展两性离子水凝胶在各种组织工程应用中的多功能性和应用方面具有很大的潜力。

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Zwitterionic Granular Hydrogel for Cartilage Tissue Engineering

Zwitterionic hydrogels have high potential for cartilage tissue engineering due to their ultra-hydrophilicity, nonimmunogenicity, and superior antifouling properties. However, their application in this field has been limited so far, due to the lack of injectable zwitterionic hydrogels that allow for encapsulation of cells in a biocompatible manner. Herein, a novel strategy is developed to engineer cartilage employing zwitterionic granular hydrogels that are injectable, self-healing, in situ crosslinkable and allow for direct encapsulation of cells with biocompatibility. The granular hydrogel is produced by mechanical fragmentation of bulk photocrosslinked hydrogels made of zwitterionic carboxybetaine acrylamide (CBAA), or a mixture of CBAA and zwitterionic sulfobetaine methacrylate (SBMA). The produced microgels are enzymatically crosslinkable using horseradish peroxidase, to quickly stabilize the construct, resulting in a microporous hydrogel. Encapsulated human primary chondrocytes are highly viable and able to proliferate, migrate, and produce cartilaginous extracellular matrix (ECM) in the zwitterionic granular hydrogel. It is also shown that by increasing hydrogel porosity and incorporation of SBMA, cell proliferation and ECM secretion are further improved. This strategy is a simple and scalable method, which has high potential for expanding the versatility and application of zwitterionic hydrogels for diverse tissue engineering applications.

更新日期：2023-07-27

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