Chemical Engineering Journal ( IF 13.3 ) Pub Date : 2023-07-26 , DOI: 10.1016/j.cej.2023.145038
Bo Liu , Siyu Lu , Kun Yang , Xuechen Dou , Xiangling Feng , Haoran Cui , Fan Li , Feng Tian
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Procalcitonin (PCT) is a crucial biomarker for diagnosing bacterial infections in clinical settings. Here, a biosensing system utilizing a fluorescence amplification system of nanocapsules and magnetic carbon dots was developed. The system incorporated two PCT sensing strategies: highly sensitive immunomagnetic separation and rapid homogeneous immunoassay. In these strategies, nanocapsules modified with antibodies function as immunosensors and energy transmitters, while magnetic carbon dots modified with antibodies serve as capture probes and immunosensors. The immunomagnetic separation analysis strategy utilized immunonanocapsules as sensors and immunomagnetic carbon dots as capture probes. This strategy demonstrated the capability to achieve ultrasensitive trace detection of PCT within the range of 1–1000 pg/mL, following normal immunoreactivity and magnetic separation. The limit of detection to 0.3 pg/mL (serum) and 0.33 pg/mL (plasma). Via the fluorescence resonance energy transfer principle, the homogeneous immunoassay strategy used immunonanocapsules as energy transmitters and immunomagnetic carbon dots as sensors. This enabled direct and rapid detection of PCT in a wide range of 0–100 ng/mL in diluted serum, with a limit of detection of 0.41 ng/mL. Both strategies demonstrated high recoveries (93.5%-104.6% and 98.4%-103.8%) and exhibited reliability in achieving accurate quantitative PCT detection. Such a novel analytical approach to PCT may broaden the potential uses of carbon dots-based immunofluorescent sensing. Furthermore, it was direct implications for ultrasensitive and rapid point-of-care detection of various serum biomarkers in clinical settings.
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使用碳点封装纳米胶囊和磁性碳点作为偶联标记快速灵敏检测降钙素原的两种策略
降钙素原 (PCT) 是临床诊断细菌感染的重要生物标志物。这里,开发了一种利用纳米胶囊和磁性碳点荧光放大系统的生物传感系统。该系统结合了两种 PCT 传感策略:高灵敏免疫磁性分离和快速均相免疫测定。在这些策略中,用抗体修饰的纳米胶囊充当免疫传感器和能量发射器,而用抗体修饰的磁性碳点充当捕获探针和免疫传感器。免疫磁性分离分析策略利用免疫纳米胶囊作为传感器,免疫磁性碳点作为捕获探针。该策略展示了在 1–1000 pg/mL 范围内实现 PCT 超灵敏痕量检测的能力,在正常的免疫反应和磁力分离之后。检测限为 0.3 pg/mL(血清)和 0.33 pg/mL(血浆)。通过荧光共振能量转移原理,均相免疫分析策略使用免疫纳米胶囊作为能量传输器,免疫磁性碳点作为传感器。这使得能够直接快速检测稀释血清中 0-100 ng/mL 范围内的 PCT,检测限为 0.41 ng/mL。两种策略均表现出高回收率(93.5%-104.6% 和 98.4%-103.8%),并且在实现准确定量 PCT 检测方面表现出可靠性。这种新颖的 PCT 分析方法可能会拓宽基于碳点的免疫荧光传感的潜在用途。此外,