甲基-CpG结合域蛋白的作用是调节水稻DNA上环丁烷嘧啶二聚体的修复。,Scientific Reports

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甲基-CpG结合域蛋白的作用是调节水稻DNA上环丁烷嘧啶二聚体的修复。
Scientific Reports ( IF 3.8 ) Pub Date : 2016-10-03 , DOI: 10.1038/srep34569
Changxun Fang , Weisi Chen , Chengxun Li , Xin Jian , Yingzhe Li , Hongmei Lin , Wenxiong Lin

UVB辐射导致在活生物体的DNA上形成环丁烷嘧啶二聚体（CPD）。这项研究发现，硅吸收基因Lsi1的过表达减少了水稻中CPD的积累，而CPD的积累得益于光裂解酶的重新激活。脱氧核糖嘧啶嘧啶光解酶（Os10g0167600）的转录丰度通常与水稻的硅含量相关，并且发现Os10g0167600的上调在UVB处理过的Lsi1-过表达（Lsi1-OX）水稻中最高。发现反式作用因子甲基CpG结合域蛋白（OsMeCP）与Os10g0167600的顺式元素相互作用。OsMeCP的核酸位置有效地实现了转录调控。与WT相比，Lsi1-OX水稻的OsMeCP水平较低，而Lsi1-RNAi系的OsMeCP水平较高。在高硅酸盐浓度的溶液中培养的水稻也显示出较少的OsMeCP丰度。与WT中相比，OsMeCP的过表达导致较低的Os10g0167600转录本水平和较高的CPD含量，但在OsMeCP-RNAi品系中则相反。这些发现表明，OsMeCP充当硅的负调节剂，并且可以介导Os10g0167600的转录抑制，从而抑制参与CPD修复的光裂解酶的光活化。

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Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA.

UVB radiation causes cyclobutane pyrimidine dimers (CPDs) to form on the DNA of living organisms. This study found that overexpression of the silicon absorbance gene Lsi1 reduced the accumulation of CPDs in rice, which profited from the reactivation by photolyase. The transcript abundance of deoxyribodipyrimidine photolyase (Os10g0167600) was generally correlated with the silicon content of the rice, and the up-regulation of Os10g0167600 was found to be highest in the UVB-treated Lsi1-overexpressed (Lsi1-OX) rice. A trans-acting factor, methyl-CpG binding domain protein (OsMeCP), was found to interact with the cis-element of Os10g0167600. The nucleic location of OsMeCP effectively enabled the transcriptional regulation. Compared with the WT, the level of OsMeCP was lower in the Lsi1-OX rice but higher in the Lsi1-RNAi line. Rice cultured in a high silicate-concentration solution also exhibited less OsMeCP abundance. Overexpression of OsMeCP led to lower Os10g0167600 transcript levels and a higher CPD content than in the WT, but the reverse was true in the OsMeCP-RNAi line. These findings indicate that OsMeCP acts as a negative regulator of silicon, and can mediate the repression of the transcription from Os10g0167600, which inhibits the photoreactivation of the photolyase involved in the repair of CPDs.

更新日期：2016-10-05

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