Herein, a novel peroxidase-like (POD-like) nanozyme Cu/B-NC was synthesized. The Cu and B co-doped nanozyme Cu/B-NC has competitive POD-like activity but negligible oxidase-like (OXD-like) activity, which is proved to partly benefit from the doping of boron atom. The catalytic activity of Cu/B-NC is high with great affinity for TMB and H₂O₂ and high reaction velocity. Cu/B-NC was utilized to catalyze the condensation of phenolic substance epinephrine (EP) and 4-aminoantipyrine (4-AAP) to form colored quinone imine in the presence of H₂O₂. UV–vis absorbance of quinone imine at 492 nm was used for EP determination. Catalase (CAT) could decompose H₂O₂, so CAT could also be quantified through absorbance variation. The linear ranges of colorimetric detection for EP and CAT were 2–100 μM and 1–30 U mL⁻¹, respectively. The limits of detection (LODs) for EP and CAT were 0.97 μM and 0.57 U mL⁻¹, respectively. The practicability of this sensing platform was further validated by successful application in actual samples.

在此，合成了一种新型过氧化物酶样（POD 样）纳米酶 Cu/B-NC。Cu和B共掺杂纳米酶Cu/B-NC具有竞争性的POD样活性，但可以忽略不计的氧化酶样（OXD样）活性，这被证明部分受益于硼原子的掺杂。Cu/B-NC催化活性高，对TMB和H ₂ O ₂亲和力大，反应速度快。利用Cu/B-NC在H ₂ O _2存在下催化酚类物质肾上腺素(EP)和4-氨基安替比林(4-AAP)缩合生成有色醌亚胺。醌亚胺在 492 nm 处的紫外可见吸光度用于 EP 测定。过氧化氢酶(CAT)可以分解H ₂ O ₂，​​因此CAT也可以通过吸光度变化来定量。EP和CAT比色检测的线性范围分别为2-100 μM和1-30 U mL ^-1。EP和CAT的检测限(LOD)分别为0.97μM和0.57UmL ^-1。通过实际样品的成功应用，进一步验证了该传感平台的实用性。