Site-Specific m6A Erasing via Conditionally Stabilized CRISPR-Cas13b Editor,Angewandte Chemie International Edition

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Site-Specific m6A Erasing via Conditionally Stabilized CRISPR-Cas13b Editor
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2023-07-25 , DOI: 10.1002/anie.202309291
Ying Xu ₁ , Yufan Wang ₁ , Fu-Sen Liang ₁

Affiliation

A conditional m⁶A erasing platform was developed. The FKBP*-dCas13b-ALKBH5 fusion protein can only be stabilized by Shield-1 to achieve site-specific m⁶A erasing. The removal of Shield-1 causes rapid degradation and release of the dCas13b-m⁶A eraser from target RNAs, while the reversal of m⁶A editing is much slower, providing an opportunity to study the m⁶A with minimal steric interference from the bulky dCas13b fusion proteins.

中文翻译：

通过条件稳定的 CRISPR-Cas13b 编辑器进行位点特异性 m6A 擦除

开发了一个有条件的 m⁶A 擦除平台。FKBP*-dCas13b-ALKBH5 融合蛋白只能通过 Shield-1 稳定，以实现位点特异性 m⁶A 擦除。去除 Shield-1 导致靶 RNA 中 dCas13b-m⁶A 擦除子的快速降解和释放，而 m⁶A 编辑的逆转要慢得多，这为研究 m⁶A 提供了机会，并且来自大体积 dCas13b 融合蛋白的空间干扰最小。

更新日期：2023-07-25

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