DNA intercalation makes possible superior-gain organic photoelectrochemical transistor detection,Biosensors and Bioelectronics

当前位置： X-MOL 学术 › Biosens. Bioelectron. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

DNA intercalation makes possible superior-gain organic photoelectrochemical transistor detection
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2023-07-22 , DOI: 10.1016/j.bios.2023.115543
Peng Ju ₁ , Yu-Yue Zhu ₂ , Tian-Tong Jiang ₃ , Ge Gao ₄ , Shi-Liang Wang ₅ , Xing-Wu Jiang ₆ , Yi-Tong Xu ₇ , Xiao-Fan Zhai ₈ , Hong Zhou ₉ , Wei-Wei Zhao ₇

Affiliation

Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, Qingdao, 266061, China; College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, PR China.
Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; College of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, PR China.
Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, Qingdao, 266061, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, 266071, China.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.

DNA intercalation has increasingly been studied for various scenario implementations due to the diverse functions of DNA/intercalators. Nascent organic photoelectrochemical transistor (OPECT) biosensing taking place in organic electronics and photoelectrochemical bioanalysis represents a promising technological frontier in the arena. In this work, we first devise DNA intercalation-enabled OPECT for miRNA detection with a superior gain up to 17100. Intercalation of [Ru(bpy)₂dppz]²⁺ within the miRNA-initiated hybrid chain reaction (HCR)-derived duplex DNA is realized for producing anodic photocurrent upon light stimulation, causing the corresponding target-dependent alternation in gate voltage (V_G) and hence the modulated channel current (I_DS) of poly (3,4-ethylenedioxythiophene) doped with poly (styrene sulfonate) (PEDOT:PSS) under specific drain voltage (V_DS) for quantitative miRNA-21 analysis, which shows a wide linear relationship and a low detection limit of 5.5 × 10⁻¹⁵ mol L⁻¹. This study features the DNA intercalation-enabled organic electronics with superior gain and is envisaged to attract more attention to explore DNA adducts for innovative bioelectronics and biosensing, given the diverse DNA binders with multiple functions.

中文翻译：

DNA 嵌入使高增益有机光电化学晶体管检测成为可能

由于 DNA/嵌入剂的不同功能， DNA嵌入越来越多地被研究用于各种场景的实现。有机电子和光电化学生物分析中出现的新兴有机光电化学晶体管（OPECT）生物传感代表了该领域有前途的技术前沿。在这项工作中，我们首先设计了用于 miRNA 检测的 DNA 嵌入 OPECT，其增益高达 17100。[Ru(bpy) ₂ dppz]²⁺在 miRNA 引发的混合链式反应 (HCR) 衍生的双链 DNA 中嵌入实现了在光刺激下产生阳极光电流，引起栅极电压（V _{G ）}相应的目标依赖性变化，从而产生聚（苯乙烯磺酸）掺杂的聚（3,4-乙撑二氧噻吩）的调制通道电流（IDS _）(PEDOT:PSS)在特定漏极电压(V _{DS )下进行定量miRNA-21分析，显示出广泛的线性关系和5.5 × 10}^-15 mol L^-1的低检测限。这项研究以具有卓越增益的 DNA 嵌入有机电子学为特色，考虑到具有多种功能的多种 DNA 结合剂，预计将吸引更多的注意力来探索用于创新生物电子学和生物传感的 DNA 加合物。

更新日期：2023-07-26

点击分享查看原文

点击收藏

阅读更多本刊新发论文