Abstract—

Biodiversity of the bacterial communities in the digestive system of Crenomytilus grayanus inhabiting the coastal Sea of Japan waters with chronic anthropogenic pollution was investigated using metabarcoding. Apart from marine bacteria, the taxa typical under contamination with oil (Rhodobacteraceae, Corynebacteriaceae), heavy metals (Asinibacterium), and unprocessed municipal waste (Cloacibacterium, Globicatella) were revealed in the microbiota. A collection of 411 cultured heterotrophic bacterial strains isolated in the course of this study was characterized taxonomically. The intestinal microbiome of the studied mollusks was shown to have a unique composition, depending on their habitat. Ability of bacterial strains isolated from the C. grayanus digestive system to degrade various nutrient substrates (sugars, amino acids, and polysaccharides) and xenobiotics (oil hydrocarbons, bisphenol A, and atrazine) was studied. Most isolates degraded a broad range of organic substrates; 13% (54 strains) oxidized oil hydrocarbons; 1% (4 strains) oxidized bisphenol A; and 0.5% (2 strains) degraded atrazine. The possible role of the microbiome C. grayanus microbiome in symbiotic digestion and in detoxication of the mollusk is discussed.

中文翻译：

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双壳类 Crenomytilus gryanus 消化系统细菌群落的生物多样性和代谢特性

摘要——

使用元条形码技术对居住在长期受到人为污染的日本海沿海水域的灰细线菌消化系统中细菌群落的生物多样性进行了研究。除了海洋细菌外，典型的分类群还受到石油（红细菌科、棒状杆菌科）、重金属（阿尼杆菌属）和未处理的城市废物（梭菌属、 球状杆菌属）的污染。）在微生物群中被揭示。对本研究过程中分离出的 411 种培养的异养细菌菌株进行了分类学表征。研究表明，所研究的软体动物的肠道微生物组具有独特的组成，具体取决于它们的栖息地。研究了从C. Grayanus消化系统中分离的细菌菌株降解各种营养底物（糖、氨基酸和多糖）和外源物质（油烃、双酚 A 和莠去津）的能力。大多数分离物可降解多种有机底物；13%（54株）氧化油碳氢化合物；1%（4株）氧化双酚A；0.5%（2株）降解莠去津。微生物组C. Grayanus的可能作用讨论了软体动物共生消化和解毒中的微生物组。