当前位置：
X-MOL 学术
›
J. Am. Chem. Soc.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Cell Surface-Anchored Fluorescent Aptamer Sensor Enables Imaging of Chemical Transmitter Dynamics
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2012-06-04 , DOI: 10.1021/ja302551p Takeshi Tokunaga 1 , Shigeyuki Namiki 2 , Katsuhiro Yamada 1 , Takahiro Imaishi 1 , Hiroshi Nonaka 1 , Kenzo Hirose 2 , Shinsuke Sando 1, 3
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2012-06-04 , DOI: 10.1021/ja302551p Takeshi Tokunaga 1 , Shigeyuki Namiki 2 , Katsuhiro Yamada 1 , Takahiro Imaishi 1 , Hiroshi Nonaka 1 , Kenzo Hirose 2 , Shinsuke Sando 1, 3
Affiliation
|
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed the direct anchoring of the fluorescent aptamer on the cell surface while retaining its performance as a fluorescent sensor. The fast-responsive fluorescent DNA aptamer sensor, which targets adenine compounds, was anchored on the surface of brain astrocytes. Fluorescence imaging of the aptamer-anchored astrocytes enabled the real-time monitoring of release of adenine compounds as a gliotransmitter, which was synchronized with calcium wave propagation in neighboring cells.
中文翻译：
细胞表面锚定荧光适体传感器实现化学发射动力学成像
荧光适体传感器被应用于细胞外化学递质动力学的分析。我们使用了生育酚标记的适体，它允许将荧光适体直接锚定在细胞表面，同时保持其作为荧光传感器的性能。靶向腺嘌呤化合物的快速响应荧光 DNA 适体传感器固定在脑星形胶质细胞的表面。适配体锚定的星形胶质细胞的荧光成像能够实时监测作为胶质递质的腺嘌呤化合物的释放，这与邻近细胞中的钙波传播同步。
更新日期：2012-06-04
中文翻译：
细胞表面锚定荧光适体传感器实现化学发射动力学成像
荧光适体传感器被应用于细胞外化学递质动力学的分析。我们使用了生育酚标记的适体，它允许将荧光适体直接锚定在细胞表面，同时保持其作为荧光传感器的性能。靶向腺嘌呤化合物的快速响应荧光 DNA 适体传感器固定在脑星形胶质细胞的表面。适配体锚定的星形胶质细胞的荧光成像能够实时监测作为胶质递质的腺嘌呤化合物的释放，这与邻近细胞中的钙波传播同步。
京公网安备 11010802027423号