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A Reversible NIR-II Ratiometric Fluorescent Probe for Real-Time In Vivo ATP Detection
Advanced Optical Materials ( IF 8.0 ) Pub Date : 2023-07-20 , DOI: 10.1002/adom.202301144 Yunyi Liu 1 , Lu Zhang 1 , Ying Chen 1 , Haitao Sun 2 , Jiajian Chen 3 , Ahmed Mohamed Ei‐Toni 4 , Aslam Khan 4 , Zuhai Lei 5 , Fan Zhang 1
Advanced Optical Materials ( IF 8.0 ) Pub Date : 2023-07-20 , DOI: 10.1002/adom.202301144 Yunyi Liu 1 , Lu Zhang 1 , Ying Chen 1 , Haitao Sun 2 , Jiajian Chen 3 , Ahmed Mohamed Ei‐Toni 4 , Aslam Khan 4 , Zuhai Lei 5 , Fan Zhang 1
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ATP plays a prominent role in shaping the evolution of immune cell responses to injury, infection, and cancer. However, real-time monitoring of ATP levels in vivo remains a challenge, due to the lack of reliable tools that work reversibly in deep tissues. Herein, based on the Förster resonance energy transfer (FRET) strategy, a reversible ratiometric second near-infrared window (NIR-II) molecular fluorescent probe (CX-RATP) is designed and synthesized. CX-RATP exhibits a significant fluorescence ratio change with the existence of 0–10 mM ATP in vitro, which is within the range of physiological and pathological ATP concentrations. Meanwhile, the recognition process can be reversed by apyrase. It can capture the increase and decrease in ATP levels in real time before and after the treatment of acute inflammation. Besides, CX-RATP is also successfully applied to the hepatocellular carcinoma (HCC) model and achieves in situ imaging of tumor lesions. These results all provide solid proof that CX-RATP is a powerful tool for in vivo real-time ATP detection.
中文翻译：
用于实时体内 ATP 检测的可逆 NIR-II 比率荧光探针
ATP 在塑造免疫细胞对损伤、感染和癌症反应的进化过程中发挥着重要作用。然而，由于缺乏在深层组织中可逆工作的可靠工具，实时监测体内 ATP 水平仍然是一个挑战。在此，基于福斯特共振能量转移（FRET）策略，设计并合成了可逆比率第二近红外窗口（NIR-II）分子荧光探针（CX-RATP）。CX-RATP在体外随0-10 mM ATP的存在而表现出显着的荧光比率变化，该变化在生理和病理ATP浓度范围内。同时，腺苷三磷酸双磷酸酶可以逆转该识别过程。它可以实时捕捉急性炎症治疗前后ATP水平的升高和降低。除了，CX-RATP还成功应用于肝细胞癌（HCC）模型，实现肿瘤病灶的原位成像。这些结果都有力地证明了CX-RATP是体内实时ATP检测的强大工具。
更新日期：2023-07-20
中文翻译：
用于实时体内 ATP 检测的可逆 NIR-II 比率荧光探针
ATP 在塑造免疫细胞对损伤、感染和癌症反应的进化过程中发挥着重要作用。然而，由于缺乏在深层组织中可逆工作的可靠工具，实时监测体内 ATP 水平仍然是一个挑战。在此，基于福斯特共振能量转移（FRET）策略，设计并合成了可逆比率第二近红外窗口（NIR-II）分子荧光探针（CX-RATP）。CX-RATP在体外随0-10 mM ATP的存在而表现出显着的荧光比率变化，该变化在生理和病理ATP浓度范围内。同时，腺苷三磷酸双磷酸酶可以逆转该识别过程。它可以实时捕捉急性炎症治疗前后ATP水平的升高和降低。除了，CX-RATP还成功应用于肝细胞癌（HCC）模型，实现肿瘤病灶的原位成像。这些结果都有力地证明了CX-RATP是体内实时ATP检测的强大工具。
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