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Development of a Fluorescent Immunochromatographic Assay Based on Quantum Dot-Functionalized Two-Dimensional Monolayer Ti3C2 MXene Nanoprobes for the Simultaneous Detection of Influenza A Virus and SARS-CoV-2
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-07-19 , DOI: 10.1021/acsami.3c05424 Ye Liu 1 , Yue Lv 2 , Wenji Chen 1 , Xingsheng Yang 1 , Xiaodan Cheng 1 , Zhen Rong 1 , Shengqi Wang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-07-19 , DOI: 10.1021/acsami.3c05424 Ye Liu 1 , Yue Lv 2 , Wenji Chen 1 , Xingsheng Yang 1 , Xiaodan Cheng 1 , Zhen Rong 1 , Shengqi Wang 1
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Accurate and rapid detection of the influenza A virus (FluA) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can effectively control their spread. We developed a colorimetric and fluorescent dual-functional two-channel immunochromatographic assay (ICA) biosensor to simultaneously detect the above-mentioned viruses. A unique two-dimensional Ti3C2-QD immunoprobe was established by adsorbing dense quantum dots (QDs) onto the light green monostromatic Ti3C2 MXene surface, resulting in light green colorimetric and superior fluorescence signals and guaranteeing high sensitivity, stability, and excellent liquidity for ICA detection. Rapid visual screening for FluA and SARS-CoV-2 infections was applicable via a green colorimetric signal. Sensitive and quantitative detection of viruses in their early stages of infection was performed by using the fluorescence signal. Our proposed Ti3C2-QD-ICA biosensor can simultaneously detect 1 ng/mL or 2.4 pg/mL FluA and 1 ng/mL or 6.2 pg/mL SARS-CoV-2 via its colorimetric or fluorescence signals, respectively, with a short testing time (20 min), good reproducibility, specificity, and accuracy. In addition, this method demonstrated sensitivity higher than that of the conventional AuNP-based ICA method in throat swab samples. Hence, our proposed Ti3C2-QD-ICA method can be potentially applied for the rapid, ultrasensitive, and multiplex detection of respiratory viruses.
中文翻译：
开发基于量子点功能化二维单层 Ti3C2 MXene 纳米探针的荧光免疫色谱法，用于同时检测甲型流感病毒和 SARS-CoV-2
准确快速检测甲型流感病毒（FluA）和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）可以有效控制其传播。我们开发了比色和荧光双功能双通道免疫层析测定（ICA）生物传感器来同时检测上述病毒。通过将致密量子点（QD）吸附到浅绿色单层Ti 3 C 2 MXene表面上，建立了一种独特的二维Ti 3 C 2 -QD免疫探针，产生浅绿色比色和优异的荧光信号，保证了高灵敏度、稳定性、以及用于 ICA 检测的出色流动性。通过绿色比色信号可对 FluA 和 SARS-CoV-2 感染进行快速目视筛查。利用荧光信号对感染早期的病毒进行灵敏且定量的检测。我们提出的 Ti 3 C 2 -QD-ICA 生物传感器可以通过其比色或荧光信号分别同时检测 1 ng/mL 或 2.4 pg/mL FluA 和 1 ng/mL 或 6.2 pg/mL SARS-CoV-2，测试时间短（20分钟），重现性、特异性和准确性良好。此外，该方法在咽拭子样本中的灵敏度高于传统的基于 AuNP 的 ICA 方法。因此，我们提出的Ti 3 C 2 -QD-ICA方法可潜在地应用于呼吸道病毒的快速、超灵敏和多重检测。
更新日期：2023-07-19
中文翻译：
开发基于量子点功能化二维单层 Ti3C2 MXene 纳米探针的荧光免疫色谱法，用于同时检测甲型流感病毒和 SARS-CoV-2
准确快速检测甲型流感病毒（FluA）和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）可以有效控制其传播。我们开发了比色和荧光双功能双通道免疫层析测定（ICA）生物传感器来同时检测上述病毒。通过将致密量子点（QD）吸附到浅绿色单层Ti 3 C 2 MXene表面上，建立了一种独特的二维Ti 3 C 2 -QD免疫探针，产生浅绿色比色和优异的荧光信号，保证了高灵敏度、稳定性、以及用于 ICA 检测的出色流动性。通过绿色比色信号可对 FluA 和 SARS-CoV-2 感染进行快速目视筛查。利用荧光信号对感染早期的病毒进行灵敏且定量的检测。我们提出的 Ti 3 C 2 -QD-ICA 生物传感器可以通过其比色或荧光信号分别同时检测 1 ng/mL 或 2.4 pg/mL FluA 和 1 ng/mL 或 6.2 pg/mL SARS-CoV-2，测试时间短（20分钟），重现性、特异性和准确性良好。此外，该方法在咽拭子样本中的灵敏度高于传统的基于 AuNP 的 ICA 方法。因此，我们提出的Ti 3 C 2 -QD-ICA方法可潜在地应用于呼吸道病毒的快速、超灵敏和多重检测。
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