薯蓣花青素纯化、鉴定对小鼠抗氧化能力的影响,Food Science & Nutrition

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薯蓣花青素纯化、鉴定对小鼠抗氧化能力的影响
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2023-07-18 , DOI: 10.1002/fsn3.3547
Pingfei Qiu ₁ , Junpu Chen ₁ , Junlong Wu ₁ , Qin Wang ₁ , Yanrong Hu ₁ , Xiaochun Li ₁ , Huiyu Shi ₁ , Xuemei Wang ₁

Affiliation

越来越多的研究致力于寻找天然活性成分作为改善健康状况的添加剂。花青素是一种水溶性天然色素，因其良好的抗氧化活性而受到关注。在这项研究中，我们从薯蓣中纯化了花青素，鉴定了其化合物，并评估了其抗氧化特性。结果表明，经AB-8大孔树脂、Sep-Pak C18固相、LH-20 Sephadex逐级纯化后，花青素纯度分别提高到39.59±1.56%、60.18±1.97%和81.08±1.97%。超高效液相色谱串联质谱分析结果表明，芍药苷-3,5-O-二己糖苷、矮牵牛素-3-O-阿魏酰葡萄糖苷-5-O-葡萄糖苷、花青素-3-O-阿魏酰葡萄糖苷-5-O-主要化合物为葡萄糖苷、花青素-3-O-槐糖苷和矮牵牛素-3,5-O-二己糖苷。^{使用DPPH、ABTS和Fe 3+}还原能力方法评估纯化的花青素比未纯化的提取物和抗坏血酸表现出更强的抗氧化活性，而比一般花青素-3-O-葡萄糖苷的抗氧化活性弱。此外，在给予脂多糖24小时后，纯化的花青素增加了GSH-Px、总抗氧化能力和超氧化物歧化酶活性，并降低了小鼠血清中的丙二醛浓度(p < .05 ) 。综上所述，纯化后的花青素比粗提物具有更出色的抗氧化性能。本研究结果为花青素来源提供了参考，有利于薯蓣资源的利用。

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The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice

Increasing findings devote to searching for natural active compositions as additives to ameliorate health status. Anthocyanin, water-soluble natural pigment, has been concerned due to its favorable antioxidant activity. In this study, we purified anthocyanin from Dioscorea alata L., identified its compounds, and evaluated its antioxidant properties. The results indicated that the purity of anthocyanin increased to 39.59 ± 1.56%, 60.18 ± 1.97%, and 81.08 ± 1.97% after purification via AB-8 macroporous resin, Sep-Pak C18 solid phase, and LH-20 Sephadex stepwise. Ultra-performance liquid chromatography tandem mass spectrometer results indicated that paeoniflorin-3,5-O-dihexoside, petunin-3-O-feruloyl-glucoside-5-O-glucoside, cyanidin-3-O-feruloyl glucoside-5-O-glucoside, cyanidin-3-O-sophoroside, and petunin-3,5-O-dihexoside were the major compounds. The purified anthocyanin exhibited stronger antioxidant activity than the unpurified extract and ascorbic acid, whereas weaker than that of cyanidin-3-O-glucoside in general, which was assessed using DPPH, ABTS, and Fe³⁺ reducing capacity methods. Moreover, the purified anthocyanin increased GSH-Px, total antioxidant capacity, and superoxide dismutase activities and decreased malondialdehyde concentration on serum in mice after administering lipopolysaccharide for 24 h (p < .05). To summarize, the purified anthocyanin boasts more outstanding antioxidant properties than that of crude extracts. These results provide a reference with source of anthocyanin and it is conducive to use Dioscorea alata L. resources.

更新日期：2023-07-18

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