无定形磷酸钙和无定形磷酸钙羧酸盐：合成与表征,ACS Omega

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无定形磷酸钙和无定形磷酸钙羧酸盐：合成与表征
ACS Omega ( IF 3.7 ) Pub Date : 2023-07-17 , DOI: 10.1021/acsomega.3c00796
Abhishek Indurkar _{1,

2} , Rajan Choudhary _{1,

2} , Kristaps Rubenis _{1,

2} , Mansingraj Nimbalkar ₃ , Anatolijs Sarakovskis ₄ , Aldo R Boccaccini ₅ , Janis Locs _{1,

2}

Affiliation

无定形磷酸钙（ACP）是从过饱和磷酸钙溶液中沉淀出来的第一个固相。当然，ACP 是在生物矿化的初始阶段形成的，并由有机化合物稳定。已知含有羧基的有机化合物可调节羟基磷灰石的成核和结晶。因此，从仿生的角度来看，合成含有ACP（ACPC）的羧酸根离子是有价值的。通常，ACP 的合成步骤比 ACPC 少。ACP的沉淀反应很快，并受pH、温度、前体浓度、搅拌条件和反应时间的影响。由于磷酸盐的三元性质，以多步骤方法控制 pH 值变得乏味。在这里，我们开发了一种新的 ACP 和 ACPC 合成方法，并对所获得的材料进行了彻底的表征。振动光谱、核磁共振 (NMR)、X 射线光电子能谱 (XPS)、真密度、比表面积和离子释放研究的结果表明 ACP 和 ACPC 的理化性质存在差异。此外，还表征了羧基离子类型对 ACPC 理化性质的影响。所有ACP和ACPC均在无菌条件下合成，并使用MC-3T3E1细胞进行体外分析，揭示了合成的ACP和ACPC的细胞相容性，其中用柠檬酸盐合成的ACPC表现出最高的细胞活力。

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Amorphous Calcium Phosphate and Amorphous Calcium Phosphate Carboxylate: Synthesis and Characterization

Amorphous calcium phosphate (ACP) is the first solid phase precipitated from a supersaturated calcium phosphate solution. Naturally, ACP is formed during the initial stages of biomineralization and stabilized by an organic compound. Carboxylic groups containing organic compounds are known to regulate the nucleation and crystallization of hydroxyapatite. Therefore, from a biomimetic point of view, the synthesis of carboxylate ions containing ACP (ACPC) is valuable. Usually, ACP is synthesized with fewer steps than ACPC. The precipitation reaction of ACP is rapid and influenced by pH, temperature, precursor concentration, stirring conditions, and reaction time. Due to phosphates triprotic nature, controlling pH in a multistep approach becomes tedious. Here, we developed a new ACP and ACPC synthesis approach and thoroughly characterized the obtained materials. Results from vibration spectroscopy, nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), true density, specific surface area, and ion release studies have shown a difference in the physiochemical properties of the ACP and ACPC. Additionally, the effect of a carboxylic ion type on the physiochemical properties of ACPC was characterized. All of the ACPs and ACPCs were synthesized in sterile conditions, and in vitro analysis was performed using MC-3T3E1 cells, revealing the cytocompatibility of the synthesized ACPs and ACPCs, of which the ACPC synthesized with citrate showed the highest cell viability.

更新日期：2023-07-17

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