DNA 编码的生物分析用 Pt 纳米酶过氧化物酶活性的双向调节,Analytical Chemistry

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DNA 编码的生物分析用 Pt 纳米酶过氧化物酶活性的双向调节
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-07-17 , DOI: 10.1021/acs.analchem.3c01564
Wenjing Mei ₁ , Weixuanzi Huang ₁ , Xiaofeng Liu ₁ , Hongqiang Wang ₁ , Qing Wang ₁ , Xiaohai Yang ₁ , Kemin Wang ₁

Affiliation

纳米酶活性的合理调控可以通过扩展传感策略和提高传感性能来促进生化传感，但有效的调控策略的设计仍然是一个挑战。在此，开发了一种快速 DNA 编码策略，用于有效调控 Pt 纳米酶活性。有趣的是，我们发现Pt纳米酶的催化活性是序列依赖性的，并且只有在富含T的序列存在下，其过氧化物酶活性才会显着增强。由此，不同的DNA序列实现了Pt纳米酶过氧化物酶活性的双向调控。此外，DNA编码策略可以有效增强Pt纳米酶在高温、冷冻和长期储存下的稳定性。_{同时，一系列研究表明DNA的存在影响H 2} PtCl ₆前体的还原程度，进而影响Pt纳米酶的过氧化物酶活性。作为应用证明，基于Pt纳米酶系统的传感器阵列在准确辨别抗氧化剂方面表现出了优越的性能。该研究获得了DNA对Pt纳米酶的调控规律，为新型传感平台的开发提供了理论指导，也为其他纳米酶活性的调控提供了新思路。

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DNA-Encoded Bidirectional Regulation of the Peroxidase Activity of Pt Nanozymes for Bioanalysis

Rational regulation of nanozyme activity can promote biochemical sensing by expanding sensing strategies and improving sensing performance, but the design of effective regulatory strategies remains a challenge. Herein, a rapid DNA-encoded strategy was developed for the efficient regulation of Pt nanozyme activity. Interestingly, we found that the catalytic activity of Pt nanozymes was sequence-dependent, and its peroxidase activity was significantly enhanced only in the presence of T-rich sequences. Thus, different DNA sequences realized bidirectional regulation of Pt nanozyme peroxidase activity. Furthermore, the DNA-encoded strategy can effectively enhance the stability of Pt nanozymes at high temperatures, freezing, and long-term storage. Meanwhile, a series of studies demonstrated that the presence of DNA influenced the reduction degree of H₂PtCl₆ precursors, which in turn affected the peroxidase activity of Pt nanozymes. As a proof of application, the sensor array based on the Pt nanozyme system showed superior performance in the accurate discrimination of antioxidants. This study obtained the regulation rules of DNA on Pt nanozymes, which provided theoretical guidance for the development of new sensing platforms and new ideas for the regulation of other nanozyme activities.

更新日期：2023-07-17

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