In order to commercialize aqueous two-phase systems (ATPSs), not only the equilibrium data is essential, but also the knowledge of separation mechanisms, kinetics, settling time, and operational conditions are needed. Mixing duration and settling time are the most critical factors affecting separation and biomolecule partitioning in terms of economic aspects. This research aimed to find the desired conditions for separating cephalexin in an ATPS consisting of acetonitrile, glucose, and water. Firstly, the evolution of the interphase region was observed. Hereafter, to examine the effect of time on the experimental tie-lines and partition coefficient in non-equilibrium states, the settling time was varied from 2 min to 24 h. In addition, centrifugation was applied to help the separation at different time intervals and rotational speeds. The results of tie-lines slope and partitioning coefficients showed that the system approaches equilibrium after 5 h. However, using the centrifuge separation at 4000 rpm improved the separation time to 45 min, reaching 80 % of the actual partition coefficient. It can be concluded that with an acceptable tolerance in the partition coefficient, a remarkably diminished settling time is available for economic productivity in industrial units.

中文翻译：

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葡萄糖和乙腈水两相体系中头孢氨苄分配的非平衡分离时间研究

为了实现水性两相系统（ATPS）的商业化，不仅平衡数据至关重要，而且还需要分离机制、动力学、沉降时间和操作条件的知识。从经济角度来看，混合持续时间和沉降时间是影响分离和生物分子分配的最关键因素。本研究旨在寻找在由乙腈、葡萄糖和水组成的 ATPS 中分离头孢氨苄的所需条件。首先，观察相间区域的演化。此后，为了检查时间对非平衡状态下实验联络线和分配系数的影响，沉降时间从 2 分钟变化到 24 小时。此外，采用离心法以不同的时间间隔和转速来帮助分离。联络线斜率和分配系数的结果表明系统在5小时后接近平衡。而采用4000rpm离心分离将分离时间缩短至45min，达到实际分配系数的80%。可以得出结论，在分配系数的公差可接受的情况下，工业单位的经济生产率可显着缩短沉降时间。