Terpinen-4-ol, an active component of tea tree oil, exhibits broad-spectrum antimicrobial activity. However, the high volatilization of terpinen-4-ol and its nonwettability property have limited its application. Our objective was to synthesize novel nanocarriers to deliver and protect terpinen-4-ol. The polyethylene glycol (PEG)-stabilized lipid nanoparticles were prepared and characterized by scanning electron microscope, Zetasizer, and differential scanning calorimetry. These nanoparticles had an average diameter of 397 nm and a Ζ-potential of 10 mV after being modified by glycine. Results showed that homogeneous particle size, high drug loading, stability, and targeting were obtained by the nanoparticles. Liquid chromatography/mass spectrometry showed a sustained release trend from nanoparticles for terpinen-4-ol. Minimum inhibitory concentration and minimum biofilm eradication concentration were tested against Candida albicans ATCC 11231. Studies on isolated mitochondria showed the blockage of biofilm respiration and inhibition of enzyme activity. The effects can be ascribed to localization of terpinen-4-ol on the membrane of mitochondria.

中文翻译：

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萜品醇-4-ol的新型PEG稳定的脂质纳米颗粒的表征，抗生物膜和作用机理。

茶树油的活性成分Terpinen-4-ol具有广谱抗菌活性。然而，萜品四醇的高挥发性及其不可润湿性限制了其应用。我们的目标是合成新型纳米载体，以递送和保护萜品四醇。制备了聚乙二醇（PEG）稳定的脂质纳米颗粒，并通过扫描电子显微镜，Zetasizer和差示扫描量热法对其进行了表征。这些纳米颗粒在被甘氨酸修饰后具有397nm的平均直径和10mV的Z电位。结果表明，纳米颗粒获得了均一的粒径，高载药量，稳定性和靶向性。液相色谱/质谱法显示从萜烯四醇的纳米颗粒中持续释放的趋势。白色念珠菌ATCC11231。对分离的线粒体的研究表明，生物膜呼吸受到阻滞，酶活性受到抑制。这种作用可归因于萜品醇-4-醇在线粒体膜上的定位。