Bentazone (BNTZ) is a selective and efficient herbicide used in crop production worldwide. However, the persistence of BNTZ residues in the environment has led to their increasing accumulation in farmland and crops, posing a high risk to human health. To evaluate its impact on crop growth and environmental safety, a comprehensive study was conducted on BNTZ toxicity, metabolic mechanism, and resultant pathways in rice. The rice growth was compromised to the treatment with BNTZ at 0.2–0.8 mg/L (529.95–1060.05 g a.i./ha), while the activities of enzymes including SOD, POD, CAT, GST, GT, and CYP450 were elevated under BNTZ stress. A genome-wide RNA-sequencing (RNA-Seq) was performed to dissect the variation of transcriptomes and metabolic mechanisms in rice exposed to BNTZ. The degradative pathways of BNTZ in rice are involved in glycosylation, hydrolysis, acetylation, and conjugation processes catalyzed by the enzymes. Our data provided evidence that helps understand the BNTZ metabolic and detoxic mechanisms.

中文翻译：

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水稻中苯达松的多种代谢途径可能受到代谢酶的调节

苯达松 (BNTZ) 是一种选择性高效除草剂，广泛用于全球农作物生产。然而，BNTZ残留物在环境中的持久存在导致其在农田和农作物中的积累不断增加，对人类健康构成了很高的风险。为了评估其对作物生长和环境安全的影响，对BNTZ在水稻中的毒性、代谢机制和产生的途径进行了全面的研究。0.2–0.8 mg/L (529.95–1060.05 g ai/ha) BNTZ 处理水稻生长受到影响，而 SOD、POD、CAT、GST、GT 和 CYP450 等酶活性在 BNTZ 胁迫下升高。通过全基因组 RNA 测序 (RNA-Seq) 来剖析暴露于 BNTZ 的水稻中转录组和代谢机制的变化。BNTZ 在水稻中的降解途径涉及酶催化的糖基化、水解、乙酰化和缀合过程。我们的数据提供了有助于理解 BNTZ 代谢和解毒机制的证据。