Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry,Journal of the American Chemical Society

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Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-07-13 , DOI: 10.1021/jacs.3c04768
Vanessa Rück ₁ , Narendra K Mishra ₂ , Kasper K Sørensen ₂ , Mikkel B Liisberg ₁ , Ane B Sloth _{3,

4} , Cecilia Cerretani ₁ , Christian B Mollerup ₅ , Andreas Kjaer _{3,

4} , Chenguang Lou ₆ , Knud J Jensen ₂ , Tom Vosch ₁

Affiliation

DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method for linking a well-characterized DNA-AgNC to molecules of interest is presented. Three different peptides and a small protein, human insulin, were tested as labeling targets. The conjugation to the target compounds was verified by MS, HPLC, and time-resolved anisotropy measurements. Moreover, the spectroscopic properties of DNA-AgNCs were found to be unaffected by the linking reactions. For DNA-AgNC-conjugated human insulin, fluorescence imaging studies were performed on Chinese hamster ovary (CHO) cells overexpressing human insulin receptor B (hIR-B). The specific staining of the CHO cell membranes demonstrates that DNA-AgNCs are great candidates for bioimaging applications, and the proposed linking strategy is easy to implement when the DNA-AgNC structure is known.

更新日期：2023-07-13

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