Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks,Nano Letters

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Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks
Nano Letters ( IF 9.6 ) Pub Date : 2023-07-13 , DOI: 10.1021/acs.nanolett.3c00750
Juanjuan Yang _{1,

2} , Kevin Jahnke ₃ , Ling Xin ₂ , Xinxin Jing ₂ , Pengfei Zhan ₂ , Andreas Peil ₂ , Alessandra Griffo ₃ , Marko Škugor ₂ , Donglei Yang ₁ , Sisi Fan ₂ , Kerstin Göpfrich _{3,

4} , Hao Yan ₅ , Pengfei Wang ₁ , Na Liu _{2,

6}

Affiliation

Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micrometer-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.

中文翻译：

通过动态 DNA 折纸网络调节脂质膜形态

膜形态及其动态适应调节许多细胞功能，这些功能通常由膜蛋白介导。DNA 纳米技术的进步使得各种受蛋白质启发的结构和功能得以实现，并在纳米水平上进行精确控制，这为人工设计膜形态提供了一种可行的工具。在这项工作中，我们展示了一种 DNA 折纸交叉 (DOC) 结构，该结构可以锚定在巨大的单层囊泡 (GUV) 上，然后聚合成微米级可重构的一维 (1D) 链或二维 (2D) 晶格。这种基于 DNA 折纸的网络可以通过 DNA 燃料在左手 (LH) 和右手 (RH) 构象之间切换，并在重塑 GUV 膜曲率方面表现出强大的功效。这项工作揭示了设计分层组装的动态 DNA 系统，以对合成细胞进行可编程调制，以实现有用的应用。

更新日期：2023-07-13

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