用于长效化学发光和高灵敏肿瘤标志物测定的磁芯-大介孔卫星免疫传感器的构建,Small

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用于长效化学发光和高灵敏肿瘤标志物测定的磁芯-大介孔卫星免疫传感器的构建
Small ( IF 13.0 ) Pub Date : 2023-07-12 , DOI: 10.1002/smll.202304631
Shude Liu ₁ , Juan Li _{1,

2} , Yidong Zou ₁ , Yongjian Jiang ₃ , Limin Wu ₄ , Yonghui Deng ₁

Affiliation

化学发光免疫分析具有较高的灵敏度和信噪比，在疾病的早期诊断和动态监测中受到广泛关注。然而，传统的闪光式化学发光信号（<5 s）的采集严重依赖于自动采样和读数仪器。本文设计了一种新型核心卫星多功能化学发光免疫传感器，用于有效富集和高灵敏度测定癌症生物标志物癌胚抗原（CEA），具有增强且持久的输出信号，可以通过简单的微孔板读板仪方便地记录。合成抗CEA单克隆抗体2（Ab2）修饰的Fe ₃ O ₄ @SiO ₂微球（Fe ₃ O ₄ @SiO ₂ -Ab2，直径370 nm）作为核心，用于选择性捕获和富集溶液中的目标CEA，并合成抗人CEA单克隆抗体1（Ab1）和辣根过氧化物酶（HRP）共固定化树枝状大介孔二氧化硅纳米球（MSNs-HRP/Ab1，直径80 nm，孔径：17 nm）作为卫星，用于高效免疫识别和信号放大。设计的核心卫星磁性化学发光免疫传感器具有0.01−20 ng mL ^-1的宽线性范围和3.0 pg mL ^-1的低检测限，可方便、高度特异性和灵敏地测定人血清中的CEA。这种核心卫星化学发光免疫传感器有望成为体外检测各种生物标志物的有力工具，克服传统化学发光严重依赖昂贵且笨重的自动化仪器的缺陷，将化学发光分析普及到基层医疗机构和边远地区。

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Construction of Magnetic Core–Large Mesoporous Satellite Immunosensor for Long-Lasting Chemiluminescence and Highly Sensitive Tumor Marker Determination

Chemiluminescence immunoassay exhibits high sensitivity and signal-to-noise ratio, thus attracting great attention in the early diagnosis and dynamic monitoring of diseases. However, the collection of conventional flash-type chemiluminescence signal (<5 s) relies heavily on automatic sampling and reading instrument. Herein, a novel core-satellite multifunctional chemiluminescence immunosensor is designed for the efficient enrichment and highly sensitive determination of cancer biomarker carcinoembryonic antigen (CEA) with enhanced and long-lasting output signal that can be conveniently recorded by a simple microplate plate reading instrument. Anti-CEA monoclonal antibody 2 (Ab2) modified Fe₃O₄@SiO₂ microspheres (Fe₃O₄@SiO₂-Ab2, 370 nm in diameter) are synthesized as the core for selectively capturing and enriching target CEA in solution, and anti-human CEA monoclonal antibody 1 (Ab1) and horseradish peroxidase (HRP) co-immobilized dendritic large-mesoporous silica nanospheres (MSNs-HRP/Ab1, 80 nm in diameter, pore size: 17 nm) are synthesized as the satellite for efficient immunological recognition and signal amplification. The as-designed core-satellite magnetic chemiluminescence immunosensors exhibit a broad linear range of 0.01−20 ng mL⁻¹ and a low detection limit of 3.0 pg mL⁻¹ for the convenient, highly specific, and sensitive determination of CEA in human serum. Such core-satellite chemiluminescence immunosensors are expected to act as a powerful tool for in vitro detection of various biomarkers, overcome the defect of conventional chemiluminescence relying heavily on expensive and bulky automatic instruments and popularize chemiluminescence analysis to primary medical institutions and remote areas.

更新日期：2023-07-12

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