基于Co3O4双酶活性的抗氧化剂识别比色传感器阵列,Analyst

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基于Co3O4双酶活性的抗氧化剂识别比色传感器阵列
Analyst ( IF 3.6 ) Pub Date : 2023-07-11 , DOI: 10.1039/d3an00939d
Pingping Hao ₁ , Zhenchao Liu ₁ , Zhiwei Wang ₁ , Min Xie ₂ , Qingyun Liu ₁

Affiliation

抗氧化剂被认为是监测人类健康的重要化合物。_{在这项工作中，使用Co 3} O ₄纳米花的类氧化酶（OXD）和类过氧化物酶（POD）活性作为传感元件，以及基底3,3',5,5'-开发了比色传感器阵列。四甲基联苯胺二盐酸盐 (TMB)，作为信号读取器，有效识别不同的抗氧化剂。_{在Co 3} O ₄存在下，无色TMB在H ₂ O ₂存在和不存在下均可不同程度地氧化成蓝色oxTMB 。有趣的是，添加抗氧化剂后，由于TMB和抗氧化剂竞争结合，传感器阵列表现出交叉反应，并且观察到颜色和吸光度的不同变化。在传感器阵列上获得不同的比色响应，并通过线性判别分析 (LDA) 进行识别。LDA结果表明，该传感器阵列可用于区分7种不同浓度（即10、20、 30、50、100、200 和 250 nM。确定了不同浓度的抗氧化剂和混合抗氧化剂的比例。这证明了传感器阵列在诊断和食品监测中的潜在应用。

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Colorimetric sensor arrays for antioxidant recognition based on Co3O4 dual-enzyme activities

Antioxidants are considered as essential compounds for monitoring human health. In this work, a colorimetric sensor array was developed using oxidase-like (OXD) and peroxidase-like (POD) activities of Co₃O₄ nanoflowers as sensing elements, together with a substrate, 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB), as a signal reader to effectively identify different antioxidants. In the presence of Co₃O₄, colorless TMB can be oxidized into blue oxTMB to different degrees in the presence and absence of H₂O₂. Interestingly, after the addition of antioxidants, the sensor array showed cross-reactions, and different changes in color and absorbance were observed, as TMB and antioxidants competed for binding. Different colorimetric responses were obtained on the sensor array and identified by linear discriminant analysis (LDA). The LDA result indicated that the sensor array can be used to distinguish 4 antioxidants, namely, dopamine (DA), glutathione (GSH), ascorbic acid (AA), and cysteine (Cys) at seven different concentrations, namely, 10, 20, 30, 50, 100, 200, and 250 nM. Different concentrations of antioxidants and proportions of mixed antioxidants were determined. This demonstrates the potential application of sensor arrays in diagnosis and food monitoring.

更新日期：2023-07-11

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