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MOF-On-MOF Dual Enzyme-Mimic Nanozyme with Enhanced Cascade Catalysis for Colorimetric/Chemiluminescent Dual-Mode Aptasensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-07-10 , DOI: 10.1021/acs.analchem.3c01905 Huining Chai 1, 2 , Kun Yu 2 , Yiming Zhao 1 , Ziyan Zhang 1 , Shasha Wang 1 , Chaonan Huang 1 , Xueji Zhang 3 , Guangyao Zhang 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-07-10 , DOI: 10.1021/acs.analchem.3c01905 Huining Chai 1, 2 , Kun Yu 2 , Yiming Zhao 1 , Ziyan Zhang 1 , Shasha Wang 1 , Chaonan Huang 1 , Xueji Zhang 3 , Guangyao Zhang 2
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A novel metal–organic framework (MOF)-on-MOF dual enzyme-mimic nanozyme was designed as enhanced cascade signal amplification for the colorimetric and chemiluminescent (CL) dual-mode aptasensing. The MOF-on-MOF hybrid is composed of MOF-818 with catechol oxidase-like activity and iron porphyrin MOF [PMOF(Fe)] with peroxidase-like activity, called MOF-818@PMOF(Fe). MOF-818 can catalyze the 3,5-di-tert-butylcatechol substrate and produce H2O2 in situ. Subsequently, PMOF(Fe) catalyzes H2O2 to produce reactive oxygen species, which oxidize 3,3′,5,5′-tetramethylbenzidine or luminol to produce color or luminescence. Thanks to the nano-proximity effect and the confinement effect, the efficiency of the biomimetic cascade catalysis is greatly improved, which in turn generates enhanced colorimetric and CL signals. Taking the detection of chlopyrifos as an example, the prepared dual enzyme-mimic MOF nanozyme is combined with the aptamer with specific recognition ability to develop a colorimetric/CL dual-mode aptasensor for highly sensitive and selective detection of chlorpyrifos. The proposed MOF-on-MOF dual nanozyme-enhanced cascade system may provide a new pathway for the further development of a biomimetic cascade sensing platform.
中文翻译：
MOF-On-MOF 双酶模拟纳米酶，具有增强级联催化作用，用于比色/化学发光双模式适体传感
设计了一种新型金属有机框架（MOF）-on-MOF双酶模拟纳米酶，用于比色和化学发光（CL）双模式适体传感的增强级联信号放大。MOF-on-MOF杂化物由具有类儿茶酚氧化酶活性的MOF-818和具有类过氧化物酶活性的铁卟啉MOF[PMOF(Fe)]组成，称为MOF-818@PMOF(Fe)。MOF-818可以催化3,5-二叔丁基儿茶酚底物并原位产生H 2 O 2 。随后，PMOF(Fe)催化H 2 O 2产生活性氧，氧化3,3',5,5'-四甲基联苯胺或鲁米诺产生颜色或发光。由于纳米邻近效应和限域效应，仿生级联催化效率大大提高，从而产生增强的比色和化学发光信号。以毒死蜱的检测为例，将制备的双酶模拟MOF纳米酶与具有特异性识别能力的适配体结合，开发出比色/CL双模式适配体传感器，用于高灵敏、选择性检测毒死蜱。所提出的MOF-on-MOF双纳米酶增强级联系统可能为仿生级联传感平台的进一步发展提供新途径。
更新日期：2023-07-10
中文翻译：
MOF-On-MOF 双酶模拟纳米酶，具有增强级联催化作用，用于比色/化学发光双模式适体传感
设计了一种新型金属有机框架（MOF）-on-MOF双酶模拟纳米酶，用于比色和化学发光（CL）双模式适体传感的增强级联信号放大。MOF-on-MOF杂化物由具有类儿茶酚氧化酶活性的MOF-818和具有类过氧化物酶活性的铁卟啉MOF[PMOF(Fe)]组成，称为MOF-818@PMOF(Fe)。MOF-818可以催化3,5-二叔丁基儿茶酚底物并原位产生H 2 O 2 。随后，PMOF(Fe)催化H 2 O 2产生活性氧，氧化3,3',5,5'-四甲基联苯胺或鲁米诺产生颜色或发光。由于纳米邻近效应和限域效应，仿生级联催化效率大大提高，从而产生增强的比色和化学发光信号。以毒死蜱的检测为例，将制备的双酶模拟MOF纳米酶与具有特异性识别能力的适配体结合，开发出比色/CL双模式适配体传感器，用于高灵敏、选择性检测毒死蜱。所提出的MOF-on-MOF双纳米酶增强级联系统可能为仿生级联传感平台的进一步发展提供新途径。
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