Pestalotiopsis sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of P. kenyana on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high efficiency, low toxicity, and environmental friendliness, which can prevent fungal diseases on a variety of crops. In this study, the effect of PCA on the morphological, physiological, and molecular characteristics of P. kenyana has been investigated, and the potential antifungal mechanism of PCA against P. kenyana was also explored. We applied PCA on P. kenyana in vitro and in vivo to determine its inhibitory effect on PCA. It was found that PCA was highly efficient against P. kenyana, with EC50 around 2.32 μg/mL, and the in vivo effect was 57% at 14 μg/mL. The mechanism of PCA was preliminarily explored by transcriptomics technology. The results showed that after the treatment of PCA, 3613 differential genes were found, focusing on redox processes and various metabolic pathways. In addition, it can also cause mycelial development malformation, damage cell membranes, reduce mitochondrial membrane potential, and increase ROS levels. This result expanded the potential agricultural application of PCA and revealed the possible mechanism against P. kenyana.

中文翻译：

---

吩嗪-1-羧酸对肯尼亚多盘拟盘菌的抗真菌机制

多拟盘拟盘菌属 是一类重要的植物病原真菌，可侵染多种农作物。我们已经证明P. kenyana对杨梅叶具有致病性并引起杨梅枯萎病。吩嗪-1-甲酸（PCA）具有高效、低毒、环境友好的特点，可防治多种作物的真菌病害。本研究探讨了PCA对肯尼亚P.kenyana形态、生理和分子特性的影响，并探讨了PCA对肯尼亚P.kenyana的潜在抗真菌机制。我们在体外和体内对肯尼亚 P. kenyana 应用 PCA，以确定其对 PCA 的抑制作用。结果发现，PCA对肯尼亚疟原虫高效，EC50约为2.32 μg/mL，14 μg/mL时体内效果为57%。利用转录组学技术初步探讨了PCA的作用机制。结果显示，经过PCA处理后，发现了36​​13个差异基因，重点关注氧化还原过程和各种代谢途径。此外，还可引起菌丝发育畸形、细胞膜损伤、线粒体膜电位降低、ROS水平升高等。这一结果扩大了 PCA 的潜在农业应用，并揭示了对抗肯尼亚 P. kenyana 的可能机制。