2-羟基-4-甲氧基苯甲醛对金黄色葡萄球菌的抗菌活性及其可能机制。,Journal of Applied Microbiology

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2-羟基-4-甲氧基苯甲醛对金黄色葡萄球菌的抗菌活性及其可能机制。
Journal of Applied Microbiology ( IF 3.2 ) Pub Date : 2023-07-04 , DOI: 10.1093/jambio/lxad144
Arunachalam Kannappan ₁ , Ravi Jothi ₂ , Xiaorong Tian ₁ , Shunmugiah Karutha Pandian ₂ , Shanmugaraj Gowrishankar ₂ , Shi Chunlei ₁

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AIM 金黄色葡萄球菌引起多种复杂的感染。尽管对新型抗菌药物的开发进行了数十年的研究，耐甲氧西林金黄色葡萄球菌 (MRSA) 仍然是一个全球健康问题。因此，迫切需要找到有效的天然抗菌化合物作为抗菌剂的替代品。鉴于此，本工作揭示了从印度半地霉中分离出的2-羟基-4-甲氧基苯甲醛（HMB）对金黄色葡萄球菌的抗菌功效和作用机制。方法和结果评估了 HMB 的抗菌活性。HMB 对金黄色葡萄球菌的最低抑菌浓度 (MIC) 为 1024 µg ml-1，最低杀菌浓度为 2 × MIC。结果通过点测定、时间灭活和生长曲线分析进行验证。此外，HMB 处理增加了 MRSA 细胞内蛋白质和核酸含量的释放。使用 SEM 分析、β-半乳糖苷酶活性以及碘化丙啶和罗丹明 123 染料的荧光强度评估细菌细胞结构形态的其他实验表明，细胞膜是 HMB 阻碍金黄色葡萄球菌生长的靶标之一。此外，成熟的生物膜根除试验表明，在测试浓度下，HMB 可以去除近 80% 的 MRSA 预先形成的生物膜。此外，发现 HMB 治疗与四环素治疗相结合可使 MRSA 细胞敏感。结论本研究表明，HMB 是一种有前景的化合物，具有抗菌和抗生物膜活性，可以作为开发新的抗 MRSA 抗菌药物的先导结构。

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Antibacterial activity of 2-hydroxy-4-methoxybenzaldehyde and its possible mechanism against Staphylococcus aureus.

AIM Staphylococcus aureus causes several complicated infections. Despite decades of research on developing new antimicrobials, methicillin-resistant S. aureus (MRSA) remains a global health problem. Hence, there is a dire need to identify potent natural antibacterial compounds as an alternative to antimicrobials. In this light, the present work divulges the antibacterial efficacy and the action mechanism of 2-hydroxy-4-methoxybenzaldehyde (HMB) isolated from Hemidesmus indicus against S. aureus. METHODS AND RESULTS Antimicrobial activity of HMB was assessed. HMB exhibited 1024 µg ml-1 as the minimum inhibitory concentration (MIC) and 2 × MIC as the minimum bactericidal concentration against S. aureus. The results were validated by spot assay, time kill, and growth curve analysis. In addition, HMB treatment increased the release of intracellular proteins and nucleic acid contents from MRSA. Additional experiments assessing the structural morphology of bacterial cells using SEM analysis, β-galactosidase enzyme activity, and the fluorescence intensities of propidium iodide and rhodamine123 dye divulged that the cell membrane as one of the targets of HMB to hinder S. aureus growth. Moreover, the mature biofilm eradication assay revealed that HMB dislodged nearly 80% of the preformed biofilms of MRSA at the tested concentrations. Further, HMB treatment was found to sensitize MRSA cells upon combining tetracycline treatment. CONCLUSIONS The present study suggests that HMB is a promising compound with antibacterial and antibiofilm activities and could act as a lead structure for developing new antibacterial drugs against MRSA.

更新日期：2023-07-08

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