一氧化氮介导的凯氏带的早期分化是拟南芥低钾条件下有效钾运输所必需的,The Plant Journal

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一氧化氮介导的凯氏带的早期分化是拟南芥低钾条件下有效钾运输所必需的
The Plant Journal ( IF 6.2 ) Pub Date : 2023-07-09 , DOI: 10.1111/tpj.16384
Qi Wu ₁ , Zhihang Feng ₁ , Hironaka Tsukagoshi ₂ , Miaoyan Yang ₃ , Yan Ao ₃ , Toru Fujiwara ₁ , Takehiro Kamiya ₁

Affiliation

卡斯帕里带 (CS) 是由木质素制成的细胞壁修饰，可作为根内皮层中的质外体屏障，限制土壤和石碑之间的养分和水传输。CS 的形成受营养条件的影响，其生理作用已被讨论。本研究发现低 K 条件会影响 CS 渗透性、木质素沉积和MYB36 mRNA 积累。为了了解这些发现背后的机制，我们重点关注一氧化氮（NO）。NO 已知作为信号分子并参与细胞壁合成，特别是木质素的合成。然而，NO 影响木质素沉积并纠正植物根部 CS 形成的机制仍不清楚。通过将荧光观察与组织学染色相结合，我们证明了根内胚层细胞对低钾（K）条件的木质化反应是由NO通过MYB36相关的木质素聚合途径介导的。此外，我们发现 NO 通过影响 CS 正确的质外体屏障形成来维持营养稳态以适应低钾条件的显着能力。总的来说，我们的结果表明，在适应低钾条件期间，根内皮层的木质化和质外体屏障的形成需要NO，这揭示了CS在低营养条件下的新生理作用，并对CS生物学做出了重大贡献。

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Early differentiation of Casparian strip mediated by nitric oxide is required for efficient K transport under low K conditions in Arabidopsis

The Casparian strip (CS) is a cell wall modification made of lignin that functions as an apoplastic barrier in the root endodermis to restrict nutrient and water transport between the soil and stele. CS formation is affected by nutritional conditions, and its physiological roles have been discussed. This study found that low K condition affects CS permeability, lignin deposition, and MYB36 mRNA accumulation. To understand the mechanism underlying these findings, we focused on nitric oxide (NO). NO is known to act as a signaling molecule and participates in cell wall synthesis, especially for lignin composition. However, the mechanism by which NO affects lignin deposition and corrects CS formation in the plant roots remains unclear. Through combining fluorescent observation with histological stains, we demonstrated that the root endodermal cell lignification response to low-potassium (K) conditions is mediated by NO through the MYB36-associated lignin-polymerizing pathway. Furthermore, we discovered the noteworthy ability of NO to maintain nutrient homeostasis for adaptation to low K conditions by affecting the correct apoplastic barrier formation of CS. Collectively, our results suggest that NO is required for the lignification and apoplastic barrier formation in the root endodermis during adaptation to low K conditions, which revealing the novel physiological roles of CS under low nutrient conditions and making a significant contribution to CS biology.

更新日期：2023-07-09

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