Solar reforming cellulosic biomass into hydrogen is an attractive research topic for sustainable biomass waste utilization and renewable energy development. To exert the advantages of enzymatic catalysis and heterogeneous photocatalysis, a glass fiber membrane integrated process that combines enzymatic hydrolysis of cellulose with sacrificial photocatalytic H₂ production from water under mild condition has been proposed as an example of integrating enzymatic catalysis and heterogeneous photocatalysis. Specifically, a low−cost Cu_0.5Ni_0.5−TiO₂ photocatalyst has been developed with presenting remarkable H₂ production performance even comparable with Pt−TiO₂ under UV light irradiation. The synergistic effect of Cu and Ni co−deposition onto TiO₂ has been found to improve the photocatalytic H₂ production. The condition for enzymatic hydrolysis of cellulose to generate glucose has been optimized in terms of reaction temperature, solution pH, types of cellulase and inorganic ions in order to obtain higher yields of glucose. To integrate the enzymatic catalysis and photocatalysis together, the glass fiber membrane with superior glucose penetration capability has been screened out. Lastly, the coupling of photocatalytic H₂ production from water based on the Cu_0.5Ni_0.5 −TiO₂ photocatalyst and enzymatic hydrolysis of cellulose has been quantitively evaluated in both mixed and membrane−separation systems. The membrane−separation system can avoid the depletion of cellulase activity induced by photocatalytic oxidation, and thus presents higher H₂ production efficiencies with apparent quantum efficiency of 3.07 % at 365 ± 10 nm irradiation in initial 5 h. This work demonstrates that inorganic membrane integrated enzymatic catalysis and photocatalysis can be a powerful tool for different potential applications.

太阳能将纤维素生物质转化为氢气是可持续生物质废物利用和可再生能源开发的一个有吸引力的研究课题。为了发挥酶催化和多相光催化的优点，提出了一种将纤维素酶水解与温和条件下牺牲光催化水产H _{2相结合的玻璃纤维膜集成工艺，作为酶催化和多相光催化集成的例子。}具体来说，我们开发了一种低成本的Cu _0.5 Ni _0.5 -TiO _{2光催化剂，其具有显着的H 2}生产性能，甚至可以与Pt−TiO _{2相媲美。}在紫外光照射下。已发现Cu和Ni共沉积到TiO ₂上的协同效应可以提高光催化H ₂的产量。从反应温度、溶液pH、纤维素酶种类和无机离子等方面对纤维素酶解生成葡萄糖的条件进行了优化，以获得更高的葡萄糖产率。将酶催化和光催化结合在一起，筛选出具有优异葡萄糖渗透能力的玻璃纤维膜。最后，基于Cu _0.5 Ni _0.5 -TiO _{2的光催化水产H 2}耦合纤维素的光催化剂和酶水解已在混合系统和膜分离系统中进行了定量评估。该膜分离系统可以避免光催化氧化引起的纤维素酶活性的损耗，从而表现出更高的H ₂生产效率，在365±10 nm照射下最初5小时的表观量子效率为3.07%。这项工作表明，无机膜集成酶催化和光催化可以成为不同潜在应用的强大工具。