芒柄花素抑制肥大细胞脱粒以改善化合物 48/80 诱导的假性过敏反应,Molecules

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芒柄花素抑制肥大细胞脱粒以改善化合物 48/80 诱导的假性过敏反应
Molecules ( IF 4.2 ) Pub Date : 2023-07-07 , DOI: 10.3390/molecules28135271
Zi-Wen Zhou ₁ , Xue-Yan Zhu ₁ , Shu-Ying Li ₁ , Si-En Lin ₁ , Yu-Han Zhu ₁ , Kunmei Ji ₁ , Jia-Jie Chen ₁

Affiliation

芒柄花素 (FNT) 是一种植物来源的异黄酮天然产物，具有抗炎、抗氧化和抗过敏特性。我们之前表明，FNT 抑制免疫球蛋白 E (IgE) 依赖的肥大细胞 (MC) 激活，但 FNT 对 IgE 独立的 MC 激活的影响尚不清楚。我们的目的是研究 FNT 对 IgE 依赖性 MC 激活和假性过敏性炎症的影响和可能的作用机制。我们使用化合物 C48/80 刺激小鼠骨髓源性肥大细胞 (BMMC) 或 RBL-2H3 细胞，通过细胞培养模型研究了 FNT 对体外 MC 脱颗粒的影响。随后我们测量了 β-己糖胺酶和组胺的释放、炎症因子的表达、细胞形态变化以及 NF-κB 信号传导的变化。我们还研究了 FNT 在几种体内过敏反应小鼠模型中的作用：C48/80 介导的被动皮肤过敏反应 (PCA)、主动全身过敏反应 (ASA) 和 2,4-二硝基苯 (DNCB) 诱导的特应性皮炎。广告）。结果表明，FNT 抑制 MC 的 IgE 依赖性脱颗粒，通过减少 β-己糖胺酶和组胺的释放以及炎症因子表达的减少来评估。此外，FNT 减少了细胞形态伸长和 F-肌动蛋白重组，并减弱了 NF-κB p65 磷酸化和 NF-κB 依赖性启动子活性。此外，在 C48/80 刺激的 PCA 和 ASA 以及 DNCB 诱导的 AD 小鼠模型中，给予 FNT 可减轻体内假过敏反应。综上所述，

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Formononetin Inhibits Mast Cell Degranulation to Ameliorate Compound 48/80-Induced Pseudoallergic Reactions

Formononetin (FNT) is a plant-derived isoflavone natural product with anti-inflammatory, antioxidant, and anti-allergic properties. We showed previously that FNT inhibits immunoglobulin E (IgE)-dependent mast cell (MC) activation, but the effect of FNT on IgE-independent MC activation is yet unknown. Our aim was to investigate the effects and possible mechanisms of action of FNT on IgE-independent MC activation and pseudoallergic inflammation. We studied the effects of FNT on MC degranulation in vitro with a cell culture model using compound C48/80 to stimulate either mouse bone marrow-derived mast cells (BMMCs) or RBL-2H3 cells. We subsequently measured β-hexosaminase and histamine release, the expression of inflammatory factors, cell morphological changes, and changes in NF-κB signaling. We also studied the effects of FNT in several in vivo murine models of allergic reaction: C48/80-mediated passive cutaneous anaphylaxis (PCA), active systemic anaphylaxis (ASA), and 2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). The results showed that FNT inhibited IgE-independent degranulation of MCs, evaluated by a decrease in the release of β-hexosaminase and histamine and a decreased expression of inflammatory factors. Additionally, FNT reduced cytomorphological elongation and F-actin reorganization and attenuated NF-κB p65 phosphorylation and NF-κB-dependent promoter activity. Moreover, the administration of FNT alleviated pseudoallergic responses in vivo in mouse models of C48/80-stimulated PCA and ASA, and DNCB-induced AD. In conclusion, we suggest that FNT may be a novel anti-allergic drug with great potential to alleviate pseudoallergic responses via the inhibition of IgE-independent MC degranulation and NF-κB signaling.

更新日期：2023-07-07

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