用于多重高分辨率空间组织病理学的膨胀真空稳定凝胶,Nature Communications

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用于多重高分辨率空间组织病理学的膨胀真空稳定凝胶
Nature Communications ( IF 14.7 ) Pub Date : 2023-07-07 , DOI: 10.1038/s41467-023-39616-w
Yunhao Bai _{1,

2} , Bokai Zhu _{1,

3} , John-Paul Oliveria _{4,

5} , Bryan J Cannon ₁ , Dorien Feyaerts ₆ , Marc Bosse ₁ , Kausalia Vijayaragavan ₁ , Noah F Greenwald ₁ , Darci Phillips ₁ , Christian M Schürch _{1,

7} , Samuel M Naik ₈ , Edward A Ganio ₆ , Brice Gaudilliere ₆ , Scott J Rodig ₈ , Michael B Miller _{8,

9,

10,

11} , Michael Angelo ₁ , Sean C Bendall ₁ , Xavier Rovira-Clavé _{1,

3} , Garry P Nolan ₁ , Sizun Jiang _{1,

11,

12,

13}

Affiliation

Department of Pathology, Stanford University, Stanford, CA, USA.
Department of Chemistry, Stanford University, Stanford, CA, USA.
Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
Department of Translational Medicine, Genentech, Inc., South San Francisco, CA, USA.
Department of Medicine, McMaster University, Hamilton, ON, Canada.
Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
Department of Pathology and Neuropathology, University Hospital and Comprehensive Cancer Center Tübingen, Tübingen, Germany.
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Division of Genetics and Genomics, Department of Pediatrics, Boston Children's Hospital, Boston, MA, USA.
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA.
Department of Pathology, Dana Farber Cancer Institute, Boston, MA, USA.

细胞组织和功能涵盖体内的多个尺度。新兴的高复数成像技术在解析亚细胞生物分子特征方面受到限制。膨胀显微镜 (ExM) 和相关技术可物理扩展样品以增强空间分辨率，但与高复数成像技术相结合以实现综合的多尺度组织生物学见解具有挑战性。在这里，我们介绍 Expand 和 comPRESS HydrOgels (ExPRESSO)，这是一种 ExM 框架，可实现高复合蛋白染色、物理膨胀和除水，同时保留横向组织膨胀。我们在多重离子束成像和成像质谱流式细胞仪平台上展示了档案临床组织样本的 ExPRESSO 成像，具有超过 40 个标记物的检测能力。ExPRESSO 在人类淋巴和脑组织档案中的应用解决了亚细胞水平的组织结构，特别是血脑屏障的结构。因此，ExPRESSO 提供了一个平台，可将水凝胶膨胀生物样本的分析兼容性扩展到质谱分析，只需对实验方案和仪器进行最小程度的修改。

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更新日期：2023-07-07

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