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Electronic Bifurcation: A New Perspective on Fe Bio-Utilization in Anaerobic Digestion of Lactate
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-07-07 , DOI: 10.1021/acs.est.3c01367 Tengyu Zhang 1, 2 , Jingxin Zhang 1 , Pengshuai Zhang 1, 2 , Jiabin Wang 1, 2 , Yiliang He 1, 2
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-07-07 , DOI: 10.1021/acs.est.3c01367 Tengyu Zhang 1, 2 , Jingxin Zhang 1 , Pengshuai Zhang 1, 2 , Jiabin Wang 1, 2 , Yiliang He 1, 2
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Anaerobic microorganisms use flavin/quinone-based electronic bifurcation (EB) to gain a survival advantage at the thermodynamic limits. However, the contribution of EB to microscopic energy and productivity in the anaerobic digestion (AD) system is unknown. This study demonstrates for the first time that under limited substrate conditions, Fe-driven EB in AD leads to a 40% increase in specific methane production and contributes to 25% ATP accumulation, by analyzing the concentration of EB enzymes such as Etf–Ldh, HdrA2B2C2, and Fd, NADH and actual Gibbs free-energy changes. Differential pulse voltammetry and electron respiratory chain inhibition experiments detected that iron enhanced electron transport in EB by accelerating the activity of flavin, Fe–S clusters, and quinone groups. Other microbial and enzyme genes with EB potential closely related to iron transport have also been found in metagenomes. The potential of EB to accumulate energy and enhance productivity in AD systems was investigated, and metabolic pathways were proposed in the study.
中文翻译：
电子分叉：乳酸厌氧消化中铁生物利用的新视角
厌氧微生物利用基于黄素/醌的电子分叉 (EB) 在热力学极限下获得生存优势。然而，EB 对厌氧消化 (AD) 系统微观能量和生产力的贡献尚不清楚。这项研究通过分析 EB 酶（如 Etf-Ldh）的浓度，首次证明在有限的底物条件下，AD 中 Fe 驱动的 EB 导致比甲烷产量增加 40%，并有助于 25% ATP 积累。 HdrA 2 B 2 C 2、Fd、NADH 和实际吉布斯自由能变化。微分脉冲伏安法和电子呼吸链抑制实验检测到铁通过加速黄素、Fe-S簇和醌基团的活性来增强EB中的电子传输。在宏基因组中也发现了与铁转运密切相关的其他具有 EB 潜力的微生物和酶基因。研究了 EB 在 AD 系统中积累能量和提高生产力的潜力，并在研究中提出了代谢途径。
更新日期：2023-07-07
中文翻译：
电子分叉：乳酸厌氧消化中铁生物利用的新视角
厌氧微生物利用基于黄素/醌的电子分叉 (EB) 在热力学极限下获得生存优势。然而，EB 对厌氧消化 (AD) 系统微观能量和生产力的贡献尚不清楚。这项研究通过分析 EB 酶（如 Etf-Ldh）的浓度，首次证明在有限的底物条件下，AD 中 Fe 驱动的 EB 导致比甲烷产量增加 40%，并有助于 25% ATP 积累。 HdrA 2 B 2 C 2、Fd、NADH 和实际吉布斯自由能变化。微分脉冲伏安法和电子呼吸链抑制实验检测到铁通过加速黄素、Fe-S簇和醌基团的活性来增强EB中的电子传输。在宏基因组中也发现了与铁转运密切相关的其他具有 EB 潜力的微生物和酶基因。研究了 EB 在 AD 系统中积累能量和提高生产力的潜力，并在研究中提出了代谢途径。
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