Journal of Applied Genetics ( IF 2.0 ) Pub Date : 2023-07-05 , DOI: 10.1007/s13353-023-00765-9 Mervat Morsy Abass Ahmed El-Gendy 1 , Shimaa M Abdel-Moniem 2 , Nabila S Ammar 2 , Ahmed Mohamed Ahmed El-Bondkly 3
Among ten metal-tolerant fungal isolates obtained from the microbiomes of detergent industry effluent, Mucor sp. NRCC6 showed the highest tolerance and an adaptive behavior toward the heavy metals Ni2+, Pb2+, Mn2+, and Zn2+. It gave the highest growth rates 0.790 ± 0.59, 0.832 ± 0.32, 0.774 ± 0.40, and 0.741 ± 1.06 mm/h along with the lowest growth inhibition 9.19, 4.37, 11.04, and 14.83% in the presence of Pb2+, Zn2+, Ni2+, and Mn2+, respectively, at a concentration of 5.0 g/L. Then, Mucor sp. NRCC6 was selected as a biotrap for the removal of these heavy metals. The optimized operating conditions were detected to be pH 6.0 for Pb2+, Zn2+, and Mn2+ and pH 5.5 for Ni2+ at 30 °C; agitation speed 150 rpm; contact time 30 min for Mn2+ and Ni2+, 30–60 min for Pb2+, and 90–180 min for Zn2+; NRCC6 biomass dosage 5.0 g/L for Ni2+ and Pb2+ and 10.0 g/L for Mn2+ and Zn2+; and initial concentration 12 mg/L of each ion in the multimetal aqueous solutions. Under these optimized conditions, the adsorption capacity for Pb2+, Ni2+, Mn2+, and Zn2+ reached 98.75, 59.25, 58.33, and 50.83%. The Langmuir isotherm was the best for describing the adsorption of Zn2+ (0.970) and Mn2+ (0.977). The Freundlich isotherm significantly giving a good fit to the adsorption of Pb2+ (0.998) while the adsorption of Ni2+ onto NRCC6 biomass can follow DKR (0.998). Furthermore, the current study revealed that Mucor sp. NRCC6 fungus is a new efficient and eco-friendly method that revealed a maximum removal of 100% for Pb2+ and Zn2+ as well as 97.39, 88.70, 78.95, 74.0, 70.22, 68.57, and 60.0% for Ni2+, Mn2+, Cd2+, Cu2+, Fe3+, As2+, and Cr6+ from the industrial wastewater, respectively.
中文翻译:
使用死毛霉生物质对水溶液进行多金属生物修复。NRCC6 源自洗涤剂生产废水
从洗涤剂工业废水的微生物组中获得的十种耐金属真菌分离株中,有毛霉属(Mucor sp.)。NRCC6对重金属Ni 2+、Pb 2+、Mn 2+和Zn 2+显示出最高的耐受性和适应性行为。在 Pb 2+、 Zn 2存在下,其生长速率最高,为 0.790 ± 0.59、0.832 ± 0.32、0.774 ± 0.40 和 0.741 ± 1.06 mm/h,同时生长抑制最低为 9.19、4.37、11.04 和 14.83%。+、Ni 2+和Mn 2+的浓度分别为5.0 g/L。然后,毛霉sp。选择 NRCC6 作为去除这些重金属的生物陷阱。经检测, 30℃下Pb 2+、Zn 2+和Mn 2+的最佳操作条件为pH 6.0,Ni 2+的最佳操作条件为pH 5.5;搅拌速度150转/分钟;接触时间 Mn 2+和 Ni 2+为 30 分钟,Pb 2+为 30–60 分钟,Zn 2+为 90–180 分钟;NRCC6生物质用量为Ni 2+和Pb 2+ 5.0 g/L ,Mn 2+和Zn 2+ 10.0 g/L ;多金属水溶液中每种离子的初始浓度为 12 mg/L。在此优化条件下,Pb 2+、Ni 2+、Mn 2+、Zn 2+的吸附量分别达到98.75%、59.25%、58.33%和50.83%。Langmuir 等温线最适合描述 Zn 2+ (0.970) 和 Mn 2+ (0.977) 的吸附。Freundlich 等温线显着地很好地拟合了 Pb 2+的吸附(0.998),而 Ni 2+到 NRCC6 生物质上的吸附可以遵循 DKR (0.998)。此外,目前的研究表明毛霉菌属。NRCC6真菌是一种高效、环保的新方法,对Pb 2+和Zn 2+的最大去除率为100%,对Ni 2+的最大去除率为97.39、88.70、78.95、74.0、70.22、68.57和60.0% ,分别来自工业废水的Mn 2+、Cd 2+、Cu 2+、Fe 3+、As 2+和Cr 6+ 。