小鼠迷走神经中节外迷走机械感受器的证据,Journal of Anatomy

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小鼠迷走神经中节外迷走机械感受器的证据
Journal of Anatomy ( IF 1.8 ) Pub Date : 2023-07-05 , DOI: 10.1111/joa.13925
Luis Leon-Mercado ₁ , Arely Tinajero ₁ , Laurent Gautron ₁

Affiliation

迷走神经传入神经元胞体位于结状神经节和颈静脉神经节中。在这项研究中，我们在 Phox2b-Cre-ZsGreen 转基因小鼠的迷走神经整体制备物中鉴定出了节外神经元。这些神经元通常沿着颈部迷走神经排列成小簇和单层。尽管不常见，但有时沿胸段和食管迷走神经观察到这些神经元。我们进行了 RNAscope 原位杂交，并证实在该转基因小鼠品系中检测到的节外神经元表达迷走神经传入标记（即Phox2b和Slc17a6）以及将其识别为潜在胃肠机械感受器的标记（即Tmc3和Glp1r）。我们还鉴定了腹腔注射氟金的野生型小鼠迷走神经中的节外神经元，从而排除了转基因小鼠特有的可能的解剖差异。在野生型小鼠中，神经节外细胞的外周蛋白呈阳性，证实了它们的神经元性质。总而言之，我们的发现揭示了以前未被发现的与迷走神经相关的节外神经元群体。展望未来，在未来与迷走神经结构和功能相关的研究中，重要的是考虑可能存在从腹部内脏传递信号的节外机械感受器。

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Evidence of extraganglionic vagal mechanoreceptors in the mouse vagus nerve

Vagal afferent neuronal somas are in the nodose and jugular ganglia. In this study, we identified extraganglionic neurons in whole-mount preparations of the vagus nerves from Phox2b-Cre-ZsGreen transgenic mice. These neurons are typically arranged in small clusters and monolayers along the cervical vagus nerve. Although infrequent, these neurons were sometimes observed along the thoracic and esophageal vagus. We performed RNAscope in situ hybridization and confirmed that the extraganglionic neurons detected in this transgenic mouse strain expressed vagal afferent markers (i.e., Phox2b and Slc17a6) as well as markers that identify them as potential gastrointestinal mechanoreceptors (i.e., Tmc3 and Glp1r). We also identified extraganglionic neurons in the vagus nerves of wild-type mice that were injected intraperitoneally with Fluoro-Gold, thereby ruling out possible anatomical discrepancies specific for transgenic mice. In wild-type mice, extraganglionic cells were positive for peripherin, confirming their neuronal nature. Taken together, our findings revealed a previously undiscovered population of extraganglionic neurons associated with the vagus nerve. Going forward, it is important to consider the possible existence of extraganglionic mechanoreceptors that transmit signals from the abdominal viscera in future studies related to vagal structure and function.

更新日期：2023-07-05

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