Hydrazine (N₂H₄) plays an important role in industrial production, but it is highly toxic, leaking or exposing it will pollute the environment and cause serious harm to human beings. Therefore, it is necessary to use a simple and effective method to detect N₂H₄ in environmental systems and organisms. Herein, a novel water-soluble fluorescent probe based on coumarin fluorophore, 2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)isoindoline-1,3-dione (C-Z1), is reported. The fluorescence intensity of the probe at 530 nm was enhanced gradually with the addition of N₂H₄, and the maximum enhancement was about 28 times. The probe has good selectivity and sensitivity, the detection limit of hydrazine hydrate is 1.48 × 10⁻⁷ M, and the response mechanism of the probe is proved by theoretical calculation and experiment. C-Z1 has been shown to detect N₂H₄ in a variety of environmental samples, including water, soil, air, cells, zebrafish and plants. In addition, C-Z1 can be made into test strips for easy portability and used for rapid quantitative detection of N₂H₄ in the field by its distinct change in fluorescence color. Thus, C-Z1 has great potential for the analysis and detection of environmental contaminants.

肼（N ₂ H ₄）在工业生产中发挥着重要作用，但它具有剧毒，泄漏或暴露会污染环境，对人类造成严重危害。因此，有必要采用一种简单有效的方法来检测环境系统和生物体中的N ₂ H ₄ 。在此，报道了一种基于香豆素荧光团2-(7-(二乙氨基)-2-氧代-2H-苯并-3-基)异吲哚啉-1,3-二酮( C-Z1 )的新型水溶性荧光探针。_{随着N 2} H ₄的加入，探针在530 nm处的荧光强度逐渐增强，最大增强约28倍。该探针具有良好的选择性和灵敏度，水合肼的检测限为1.48×10 ^-7  M，并通过理论计算和实验证明了探针的响应机理。C-Z1已被证明可以检测多种环境样品中的N ₂ H _{4 ，包括水、土壤、空气、细胞、斑马鱼和植物。}另外，C-Z1还可制成试纸条，方便携带，利用其明显的荧光颜色变化，用于现场快速定量检测N ₂ H _{4 。}因此，C-Z1在环境污染物的分析和检测方面具有巨大的潜力。