全两性离子防污表面涂料的高效可调谐三维功能化,Langmuir

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全两性离子防污表面涂料的高效可调谐三维功能化
Langmuir ( IF 3.7 ) Pub Date : 2016-09-30 00:00:00 , DOI: 10.1021/acs.langmuir.6b02622
Stefanie C. Lange ₁ , Esther van Andel _{1,

2} , Maarten M. J. Smulders ₁ , Han Zuilhof _{1,

3}

Affiliation

为了提高基于表面的（生物）传感器的灵敏度和选择性，减少由生物分子的非特异性结合（所谓的生物污染）产生的背景信号至关重要。两性离子聚合物刷已被证明是出色的防污材料。然而，出于感测目的，防污不足，但需要结合使用识别单元有效地修改刷子的可能性。迄今为止，这仅以防污性能或粘合能力为代价而实现。本文中，我们通过将两个特征整合到一个定制单体中提出了一种概念上新的方法：一种新型的基于磺基甜菜碱的两性离子单体，配有可点击的叠氮化物部分。该单体与公认的标准磺基甜菜碱单体的共聚导致高度防污的表面涂层，整个涂料中存在大量但可调节数量的可点击基团。随后通过广泛使用的应变促进炔叠氮化物咔嗒反应对叠氮基刷进行功能化，可产生完全两性离子3D功能化涂层，并具有可针对任何特定应用量身定制的识别单元。在这里，我们展示了在硅上使用生物素功能化刷的原理证明随后通过广泛使用的应变促进炔叠氮化物咔嗒反应对叠氮基刷进行功能化，可产生完全两性离子3D功能化涂层，并具有可针对任何特定应用量身定制的识别单元。在这里，我们展示了在硅上使用生物素功能化刷的原理证明随后通过广泛使用的应变促进炔叠氮化物咔嗒反应对叠氮基刷进行功能化，可产生完全两性离子3D功能化涂层，并具有可针对任何特定应用量身定制的识别单元。在这里，我们展示了在硅上使用生物素功能化刷的原理证明₃ N ₄结合了优异的防污性能和蛋白混合物中特定的抗生物素蛋白结合能力。即使叠氮化物的含量降低到1％，信噪比也比磺基甜菜碱聚合物刷的传统链端修饰显着提高。因此，这为生物传感器的开发提供了一种可行的方法，可大大提高任何表面的性能。

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Efficient and Tunable Three-Dimensional Functionalization of Fully Zwitterionic Antifouling Surface Coatings

To enhance the sensitivity and selectivity of surface-based (bio)sensors, it is of crucial importance to diminish background signals that arise from the nonspecific binding of biomolecules, so-called biofouling. Zwitterionic polymer brushes have been shown to be excellent antifouling materials. However, for sensing purposes, antifouling does not suffice but needs to be combined with the possibility to efficiently modify the brush with recognition units. So far this has been achieved only at the expense of either antifouling properties or binding capacity. Herein we present a conceptually new approach by integrating both characteristics into a single tailor-made monomer: a novel sulfobetaine-based zwitterionic monomer equipped with a clickable azide moiety. Copolymerization of this monomer with a well-established standard sulfobetaine monomer results in highly antifouling surface coatings with a large yet tunable number of clickable groups present throughout the entire brush. Subsequent functionalization of the azido brushes via widely used strain-promoted alkyne azide click reactions yields fully zwitterionic 3D-functionalized coatings with a recognition unit of choice that can be tailored for any specific application. Here we show a proof of principle with biotin-functionalized brushes on Si₃N₄ that combine excellent antifouling properties with specific avidin binding from a protein mixture. The signal-to-noise ratio is significantly improved over that of traditional chain-end modification of sulfobetaine polymer brushes, even if the azide content is lowered to 1%. This therefore offers a viable approach to the development of biosensors with greatly enhanced performance on any surface.

更新日期：2016-09-30

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