Pellicle biofilm-forming bacteria Bacillus amyloliquefaciens are the major spoilage microorganisms of soy products. Due to their inherent resistance to antibiotics and disinfectants, pellicle biofilms formed are difficult to eliminate and represent a threat to food safety. Here, we assessed linalool’s ability to prevent the pellicle of two spoilage B. amyloliquefaciens strains. The minimum biofilm inhibitory concentration (MBIC) of linalool against B. amyloliquefaciens DY1a and DY1b was 4 μL/mL and 8 μL/mL, respectively. The MBIC of linalool had a considerable eradication rate of 77.15% and 83.21% on the biofilm of the two strains, respectively. Scanning electron microscopy observations revealed that less wrinkly and thinner pellicle biofilms formed on a medium supplemented with 1/2 MBIC and 1/4 MBIC linalool. Also, linalool inhibited cell motility and the production of extracellular polysaccharides and proteins of the biofilm matrix. Furthermore, linalool exposure reduced the cell surface hydrophobicity, zeta potential, and cell auto-aggregation of B. amyloliquefaciens. Molecular docking analysis demonstrated that linalool interacted strongly with quorum-sensing ComP receptor and biofilm matrix assembly TasA through intermolecular hydrogen bonds, hydrophobic contacts, and van der Waals forces interacting with site residues. Overall, our findings suggest that linalool may be employed as a potential antibiofilm agent to control food spoilage B. amyloliquefaciens.

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芳樟醇对食品腐败解淀粉芽孢杆菌的抑菌活性及机制

皮膜生物膜形成细菌解淀粉芽孢杆菌是豆制品的主要腐败微生物。由于其对抗生素和消毒剂固有的耐药性，形成的表膜生物膜难以消除，并对食品安全构成威胁。在这里，我们评估了芳樟醇防止两种腐败解淀粉芽孢杆菌菌株的菌膜的能力。芳樟醇对解淀粉芽孢杆菌 DY1a 和 DY1b 的最低生物膜抑制浓度 (MBIC) 分别为 4 μL/mL 和 8 μL/mL。芳樟醇的MBIC对两种菌株的生物膜有相当大的根除率，分别为77.15%和83.21%。扫描电子显微镜观察表明，在补充有 1/2 MBIC 和 1/4 MBIC 芳樟醇的培养基上形成的薄膜生物膜皱纹较少且较薄。还，芳樟醇抑制细胞运动以及细胞外多糖和生物膜基质蛋白质的产生。此外，芳樟醇暴露降低了解淀粉芽胞杆菌的细胞表面疏水性、zeta 电位和细胞自动聚集。分子对接分析表明，芳樟醇通过分子间氢键、疏水接触以及与位点残基相互作用的范德华力与群体感应 ComP 受体和生物膜基质组装 TasA 发生强烈相互作用。总体而言，我们的研究结果表明，芳樟醇可作为潜在的抗生物膜剂来控制解淀粉芽孢杆菌的食物腐败。和解淀粉芽孢杆菌的细胞自动聚集。分子对接分析表明，芳樟醇通过分子间氢键、疏水接触以及与位点残基相互作用的范德华力与群体感应 ComP 受体和生物膜基质组装 TasA 发生强烈相互作用。总体而言，我们的研究结果表明，芳樟醇可作为潜在的抗生物膜剂来控制解淀粉芽孢杆菌的食物腐败。和解淀粉芽孢杆菌的细胞自动聚集。分子对接分析表明，芳樟醇通过分子间氢键、疏水接触以及与位点残基相互作用的范德华力与群体感应 ComP 受体和生物膜基质组装 TasA 发生强烈相互作用。总体而言，我们的研究结果表明，芳樟醇可作为潜在的抗生物膜剂来控制解淀粉芽孢杆菌的食物腐败。