Nuclear Magnetic Resonance (NMR) spectroscopy is a most powerful molecular characterization and quantification technique, yet two major persistent factors limit its more wide-spread applications: poor sensitivity, and intricate complex and expensive hardware required for sophisticated experiments. Here we show NMR with a single planar-spiral microcoil in an untuned circuit with hyperpolarization option and capability to execute complex experiments addressing simultaneously up to three different nuclides. A microfluidic NMR-chip in which the 25 nL detection volume can be efficiently illuminated with laser-diode light enhances the sensitivity by orders of magnitude via photochemically induced dynamic nuclear polarization (photo-CIDNP), allowing rapid detection of samples in the lower picomole range (normalized limit of detection at 600 MHz, nLOD_f,600, of 0.01 nmol Hz^1/2). The chip is equipped with a single planar microcoil operating in an untuned circuit that allows different Larmor frequencies to be addressed simultaneously, permitting advanced hetero-, di- and trinuclear, 1D and 2D NMR experiments. Here we show NMR chips with photo-CIDNP and broadband capabilities addressing two of the major limiting factors of NMR, by enhancing sensitivity as well as reducing cost and hardware complexity; the performance is compared to state-of-the-art instruments.

核磁共振 (NMR) 光谱是一种最强大的分子表征和定量技术，但两个主要的持续因素限制了其更广泛的应用：灵敏度差，以及复杂实验所需的复杂且昂贵的硬件。在这里，我们展示了在未调谐电路中使用单个平面螺旋微线圈的 NMR，该电路具有超极化选项，并且能够执行同时处理多达三种不同核素的复杂实验。微流控 NMR 芯片可以用激光二极管光有效照射 25 nL 检​​测体积，通过光化学诱导动态核极化 (photo-CIDNP) 将灵敏度提高几个数量级，从而可以快速检测较低皮摩尔范围内的样品（600 MHz 下的归一化检测限，nLOD _f,600为 0.01 nmol Hz ^1/2 ）。该芯片配备了在未调谐电路中运行的单个平面微线圈，可以同时处理不同的拉莫尔频率，从而可以进行先进的异核、双核和三核、一维和二维核磁共振实验。在这里，我们展示了具有 photo-CIDNP 和宽带功能的 NMR 芯片，通过提高灵敏度以及降低成本和硬件复杂性，解决了 NMR 的两个主要限制因素；其性能与最先进的仪器进行了比较。