Natural antimicrobials have potential to suppress the growth of foodborne pathogens. In this work, the antibacterial property and membrane-disruptive mechanism of pyrrole-2-carboxylic acid (PCA), against were elucidated. Leakage of cell constituents (K and nucleotide) and flow cytometric analysis indicated that PCA caused a significant change in the permeability and integrity of cell membrane of . Meanwhile, remarkable morphological alterations and membrane hyperpolarization of validated the disruptive ability of PCA on cell membrane. Additionally, PCA could interact with membrane protein, and cause changes in the conformation of protein. Untargeted lipidomics revealed that PCA triggered alternations in the composition and architecture of the membrane lipids mainly involved in glycerophospholipid and glycerolipid metabolism, thus leading to cell membrane dysfunction. Furthermore, PCA at concentration of 0.75 mg/mL could inhibit the contamination of in lettuce stem and beef, with a decrease of 2.4 log CFU/cm ( ＜ 0.05) for lettuce stem and 1.8 log CFU/cm ( ＜ 0.05) for beef after 10 days of storage. This work illuminates the key targets of PCA against the cell membrane of , facilitating the application of natural organic acids as candidate food preservatives in the food industry.

中文翻译：

---

深入研究吡咯-2-羧酸对单核细胞增生李斯特氏菌的抗菌活性和膜损伤机制


天然抗菌剂具有抑制食源性病原体生长的潜力。在这项工作中，阐明了吡咯-2-羧酸（PCA）的抗菌特性和膜破坏机制。细胞成分（K 和核苷酸）的泄漏和流式细胞术分析表明，PCA 导致细胞膜的通透性和完整性发生显着变化。同时，显着的形态改变和膜超极化验证了PCA对细胞膜的破坏能力。此外，PCA可以与膜蛋白相互作用，引起蛋白质构象的变化。非靶向脂质组学表明，PCA 引发主要参与甘油磷脂和甘油脂代谢的膜脂质组成和结构的改变，从而导致细胞膜功能障碍。此外，浓度为 0.75 mg/mL 的 PCA 可以抑制生菜茎和牛肉中的污染，处理后生菜茎减少了 2.4 log CFU/cm ( ＜ 0.05)，牛肉减少了 1.8 log CFU/cm ( ＜ 0.05)。储存期10天。这项工作阐明了 PCA 对抗细胞膜的关键目标，促进了天然有机酸作为候选食品防腐剂在食品工业中的应用。