The excessive or illegal use of organophosphorus pesticides (OPs) in agriculture has led to potential residue problems, making it essential to develop an effective analytical method. As reliable and rapid analytical methods, biosensors open an avenue for OP detection. However, traditional biosensors mainly depend on bio-recognition elements, consequently limiting their practical application. To address these issues, nanozymes have been widely applied because of their low cost, excellent stability, and favorable catalytic activity. This review summarizes the related research progress of nanozyme-based biosensors for OP detection in the last six years (2018–2023). The structural classification, catalytic activity, and biosensing strategy are discussed. Emphatically, this paper introduces the practical applications of nanozyme-enabled biosensors in the direction of simple operation, low cost, and on-site detection, such as optical and electrochemical biosensors. Finally, the main challenges and future trends are prospected, which could provide theoretical references for nanozyme-based biosensors in OP detection.

农业中过量或非法使用有机磷农药（OP）导致了潜在的残留问题，因此开发有效的分析方法至关重要。作为可靠、快速的分析方法，生物传感器为有机磷检测开辟了一条途径。然而，传统的生物传感器主要依赖于生物识别元件，从而限制了其实际应用。为了解决这些问题，纳米酶因其低成本、优异的稳定性和良好的催化活性而被广泛应用。本文综述了近六年（2018-2023）基于纳米酶的OP检测生物传感器的相关研究进展。讨论了结构分类、催化活性和生物传感策略。强调一下，本文介绍了纳米酶生物传感器在操作简单、成本低廉、可现场检测等方面的实际应用，如光学和电化学生物传感器。最后，对主要挑战和未来趋势进行了展望，为基于纳米酶的生物传感器在有机磷检测中提供理论参考。