Due to their distinct enzyme-like catalytic activity, iron-based nanomaterials have attracted considerable attention from researchers in the field of biomolecular detection. However, the current literature predominantly focuses on iron oxide nanozymes, with limited attention given to iron sulfide (Fe_xS_y) nanozymes. In this work, a glutathione-modified Fe_1-xS nanoparticles (Fe_1-xS NPs) with a diameter of 4–6 nm was synthesized in one-pot aqueous solution at room temperature. The resulting Fe_1-xS NPs exhibited superior peroxidase-like (POD) activity compared to natural horseradish peroxidase, indicating their potential as a promising alternative for POD applications. A sensor utilizing the POD activity of Fe_1-xS NPs was developed for detecting glucose and ascorbic-acid (AA) in soft drinks. The detection limits for glucose and AA were determined to be 37 and 5.3 μM, respectively, with broader linear ranges of 200–700 and 10–500 μM. Moreover, Fe_1-xS NPs demonstrated exceptional selectivity and anti-interference properties in detecting glucose and AA in soft drinks. Furthermore, this study proposed a smartphone-assisted colorimetric sensor based on Fe_1-xS nanozymes for rapid and specific detection of glucose and AA. This technique has the potential to be utilized in the future for convenient and efficient detection of glucose and AA.

由于其独特的类酶催化活性，铁基纳米材料引起了生物分子检测领域研究人员的广泛关注。然而，目前的文献主要集中在氧化铁纳米酶上，而对硫化铁（Fe _x S _y）纳米酶的关注有限。在这项工作中，我们在室温下在一锅水溶液中合成了直径为4-6 nm的谷胱甘肽修饰的Fe _1-x S纳米颗粒（Fe _{1-x S NPs）。}与天然辣根过氧化物酶相比，所得的 Fe _{1-x S NP 表现出优异的类过氧化物酶 (POD) 活性，表明它们作为 POD 应用的有前途的替代品的潜力。}利用 Fe 的 POD 活性的传感器_1-x S NP 专为检测软饮料中的葡萄糖和抗坏血酸 (AA) 而开发。葡萄糖和 AA 的检测限分别确定为 37 和 5.3 μM，线性范围更宽，分别为 200–700 和 10–500 μM。此外，Fe _1-x S NPs 在检测软饮料中的葡萄糖和 AA 时表现出卓越的选择性和抗干扰特性。此外，本研究提出了一种基于 Fe _1-x S 纳米酶的智能手机辅助比色传感器，用于快速、特异性检测葡萄糖和 AA。该技术有潜力在未来用于方便、高效地检测葡萄糖和 AA。