Utilization of LC–MS/MS and Drift Tube Ion Mobility for Characterizing Intact Oxidized Arachidonate-Containing Glycerophosphatidylethanolamine,Journal of the American Society for Mass Spectrometry

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Utilization of LC–MS/MS and Drift Tube Ion Mobility for Characterizing Intact Oxidized Arachidonate-Containing Glycerophosphatidylethanolamine
Journal of the American Society for Mass Spectrometry ( IF 3.1 ) Pub Date : 2023-06-27 , DOI: 10.1021/jasms.3c00083
Yulemni Morel ₁ , Jace W Jones ₁

Affiliation

Lipid peroxidation is a key component in the pathogenesis of numerous disease states, where the oxidative damage of lipids frequently leads to membrane dysfunction and subsequent cellular death. Glycerophosphoethanolamine (PE) is the second most abundant phospholipid found in cellular membranes and, when oxidized, has been identified as an executor of ferroptotic cell death. PE commonly exists in the plasmalogen form, where the presence of the vinyl ether bond and its enrichment in polyunsaturated fatty acids make it especially susceptible to oxidative degradation. This results in a multitude of oxidized products complicating identification and often requiring several analytical techniques for interpretation. In the present study, we outline an analytical approach for the structural characterization of intact oxidized products of arachidonate-containing diacyl and plasmalogen PE. Intact oxidized PE structures, including structural and positional isomers, were identified using complementary liquid chromatography techniques, drift tube ion mobility, and high-resolution tandem mass spectrometry. This work establishes a comprehensive method for the analysis of intact lipid peroxidation products and provides an important pathway to investigate how lipid peroxidation initially impacts glycerophospholipids and their role in redox biology.

中文翻译：

利用 LC-MS/MS 和漂移管离子淌度表征完整氧化的含甘油磷脂酰乙醇胺的花生四烯酸

脂质过氧化是许多疾病状态发病机制的关键组成部分，其中脂质的氧化损伤经常导致膜功能障碍和随后的细胞死亡。甘油磷酸乙醇胺 (PE) 是细胞膜中第二丰富的磷脂，当被氧化时，已被确定为铁死亡细胞死亡的执行者。PE 通常以缩醛磷脂形式存在，其中乙烯基醚键的存在及其富含的多不饱和脂肪酸使其特别容易氧化降解。这导致大量氧化产物的产生，使鉴定变得复杂，并且通常需要多种分析技术来解释。在本研究中，我们概述了一种分析方法，用于分析含花生四烯酸的二酰基和缩醛磷脂 PE 的完整氧化产物的结构表征。使用互补液相色谱技术、漂移管离子淌度和高分辨率串联质谱法鉴定了完整的氧化 PE 结构，包括结构异构体和位置异构体。这项工作建立了一种分析完整脂质过氧化产物的综合方法，并为研究脂质过氧化最初如何影响甘油磷脂及其在氧化还原生物学中的作用提供了重要途径。

更新日期：2023-06-27

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