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Efficient Intracellular MicroRNA Imaging Based on the Localized and Self-Sustainable Catalytic DNA Assembly Circuit
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-06-29 , DOI: 10.1021/acs.analchem.3c01544
Chenxia Xie 1 , Ruomeng Li 1 , Xue Gong 1 , Qingqing Zhang 1 , Yanping Zhang 1 , Zeyue Wang 1 , Xiaoqing Liu 1 , Fuan Wang 1, 2
Affiliation  

Building dynamic biological networks, especially DNA circuits, has provided a powerful prospect for exploring the intrinsic regulation processes of live cells. Nevertheless, for efficient intracellular microRNA analysis, the available multi-component circuits are constrained by their limited operating speed and efficiency due to the free diffusion of reactants. Herein, we developed an accelerated Y-shaped DNA catalytic (YDC) circuit for high-efficiency intracellular imaging of microRNA. By grafting the catalytic hairpin assembly (CHA) reactants into an integrated Y-shaped scaffold, the CHA probes were concentrated in a compact space, thus achieving high signal amplification. Profiting from the spatially confined reaction and the self-sustainably assembled DNA products, the YDC system facilitated reliable and in situ microRNA imaging in live cells. Compared with the homogeneously dispersed CHA reactants, the integrated YDC system could efficiently promote the reaction kinetics as well as the uniform delivery of CHA probes, thus providing a robust and reliable analytical tool for disease diagnosis and monitoring.

中文翻译:

基于局部且自我维持的催化 DNA 组装电路的高效细胞内 MicroRNA 成像

构建动态生物网络,特别是DNA回路,为探索活细胞的内在调控过程提供了强大的前景。然而,对于有效的细胞内 microRNA 分析,可用的多组件电路由于反应物的自由扩散而受到其有限的运行速度和效率的限制。在此,我们开发了一种加速 Y 形 DNA 催化 (YDC) 电路,用于 microRNA 的高效细胞内成像。通过将催化发夹组装(CHA)反应物接枝到集成的Y形支架中,CHA探针集中在紧凑的空间中,从而实现高信号放大。受益于空间受限反应和自我可持续组装的 DNA 产品,YDC 系统促进了可靠的原位反应活细胞中的 microRNA 成像。与均匀分散的CHA反应物相比,集成的YDC系统可以有效地促进反应动力学以及CHA探针的均匀传递,从而为疾病诊断和监测提供强大而可靠的分析工具。
更新日期:2023-06-29
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