<br>昆虫特异性RNA病毒影响棕色柑橘蚜虫（Aphis citricidus）的管心穿透活性以促进其传播,Insect Science

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昆虫特异性RNA病毒影响棕色柑橘蚜虫（Aphis citricidus）的管心穿透活性以促进其传播
Insect Science ( IF 2.9 ) Pub Date : 2023-06-26 , DOI: 10.1111/1744-7917.13242
Xin An ₁ , Qiaoying Gu ₁ , Jing Wang ₁ , Tengyu Chang ₁ , Wei Zhang ₁ , Jin-Jun Wang ₁ , Jinzhi Niu ₁

Affiliation

吸汁昆虫通常传播植物病毒，但也携带昆虫病毒，这种病毒感染昆虫而不感染植物。此类昆虫病毒对昆虫宿主生物学和生态学的影响很大程度上未知。在这里，我们鉴定了一种由棕色柑橘蚜虫（ Aphis citricidus ）携带的新型昆虫特异性病毒，我们暂时将其命名为柑橘蚜小RNA病毒（AcPV）。系统发育分析发现了AcPV和其他未指定病毒的单系簇，表明这些病毒代表了小核糖核酸病毒目中的一个新科。 AcPV 的全身感染触发了由 RNA 干扰介导的蚜虫抗病毒免疫，导致无症状耐受。重要的是，我们发现 AcPV 通过唾液腺分泌到植物的摄食部位水平传播。 AcPV影响了蚜虫取食期间的管心行为，增加了细胞间穿透所需的时间，从而促进了其以植物为中间部位在蚜虫之间的传播。基因表达结果表明，这种机制与唾液蛋白基因的转录和植物防御激素信号传导有关。总之，我们的结果表明，棕色柑橘蚜虫中 AcPV 的水平传播进化方式与昆虫媒介植物病毒循环传播的进化方式类似，从而为蚜虫中发现的昆虫特异性病毒的活性提供了新的生态视角提高对昆虫病毒生态学的认识。

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Insect-specific RNA virus affects the stylet penetration activity of brown citrus aphid (Aphis citricidus) to facilitate its transmission

Sap-sucking insects often transmit plant viruses but also carry insect viruses, which infect insects but not plants. The impact of such insect viruses on insect host biology and ecology is largely unknown. Here, we identified a novel insect-specific virus carried by brown citrus aphid (Aphis citricidus), which we tentatively named Aphis citricidus picornavirus (AcPV). Phylogenetic analysis discovered a monophyletic cluster with AcPV and other unassigned viruses, suggesting that these viruses represent a new family in order Picornavirales. Systemic infection with AcPV triggered aphid antiviral immunity mediated by RNA interference, resulting in asymptomatic tolerance. Importantly, we found that AcPV was transmitted horizontally by secretion of the salivary gland into the feeding sites of plants. AcPV influenced aphid stylet behavior during feeding and increased the time required for intercellular penetration, thus promoting its transmission among aphids with plants as an intermediate site. The gene expression results suggested that this mechanism was linked with transcription of salivary protein genes and plant defense hormone signaling. Together, our results show that the horizontal transmission of AcPV in brown citrus aphids evolved in a manner similar to that of the circulative transmission of plant viruses by insect vectors, thus providing a new ecological perspective on the activity of insect-specific viruses found in aphids and improving the understanding of insect virus ecology.

更新日期：2023-06-26

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