A novel naphthalimide-substituted calix[4]triazacrown-5 (Nap-Calix) at cone conformation was designed and synthesized to employ as a fluorescent probe, which enables the simultaneously detection of Co²⁺ and Cd²⁺ metal ions as well as dopamine (DA). ¹H-NMR, ¹³C-NMR, ESI–MS and elemental analysis techniques were carried out to characterize its structure. Cation binding property of Nap-Calix against various metal ions such as Ba²⁺, Co²⁺, Ni²⁺, Pb²⁺, Zn²⁺, and Cd²⁺ exhibited that the sensor selectively binds to Co²⁺ and Cd²⁺ metal ions with a remarkable affinity. Introduction of Co²⁺ and Cd²⁺ metal ions to a solution of Nap-Calix in DMF/water (1:1, v/v) resulted with a new emission band at 370 nm when excited at 283 nm. In addition, the fluorescence sensing affinity of the probe Nap-Calix against a catecholamine neurotransmitter (dopamine) was investigated in a wide range of concentration of DA (0–0.1 mmol L⁻¹) in 50% DMF/PBS (pH = 5.0). The fluorescence intensity of Nap-Calix, with excitation/emission peaks at 283/327 nm, is highly enhanced by DA. It was also observed that Nap-Calix exhibits excellent fluorescence behavior towards DA with a very low detection limit as 0.21 µmol L⁻¹.

设计并合成了一种新型萘酰亚胺取代的锥形构象的calix[4]triazacrown-5 ( Nap-Calix )作为荧光探针，能够同时检测Co ²⁺和Cd ²⁺金属离子以及多巴胺（DA）。采用¹ H-NMR、 ¹³ C-NMR、ESI-MS 和元素分析技术对其结构进行了表征。 Nap-Calix对Ba ²⁺ 、Co ²⁺ 、Ni ²⁺ 、Pb ²⁺ 、Zn ²⁺和Cd ²⁺等多种金属离子的阳离子结合特性表明，传感器选择性地结合Co ²⁺和Cd ^{2 +}对金属离子具有显着的亲和力。将Co ²⁺和Cd ²⁺金属离子引入Nap-Calix的DMF/水（1:1，v/v）溶液中，在283 nm 激发时在370 nm 处产生新的发射带。此外，在 50% DMF/PBS (pH = 5.0) 中的多种浓度 DA (0–0.1 mmol L ^-1 ) 中研究了探针Nap-Calix对儿茶酚胺神经递质（多巴胺）的荧光传感亲和力。 Nap-Calix的荧光强度（激发/发射峰位于 283/327 nm）可通过 DA 得到极大增强。还观察到Nap-Calix对 DA 表现出优异的荧光行为，检测限非常低，为 0.21 µmol L ^-1 。