基于卡尔文-本森-巴沙姆循环的工程异养微生物强化二氧化碳固定的最新进展,ACS Sustainable Chemistry & Engineering

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基于卡尔文-本森-巴沙姆循环的工程异养微生物强化二氧化碳固定的最新进展
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2023-06-21 , DOI: 10.1021/acssuschemeng.2c06627
Jia Feng ₁ , Ding Ma ₁ , Siyuan Gao ₁ , Yang Liao ₁ , Jiao Feng ₁ , Sheng Xu ₁ , Xin Wang ₁ , Kequan Chen ₁

Affiliation

温室气体排放引发的全球气候变化是人类面临的根本挑战。生物二氧化碳固定最近引起了广泛关注。_{卡尔文-本森-巴沙姆（CBB）循环是生物圈中的主要碳固定途径，并且由于其作为净CO 2}固定的主要途径在自然界中的突出地位和关键作用而受到最深入的研究。最近的研究表明，在大肠杆菌、酿酒酵母、扭扭甲基杆菌、马克斯克鲁维酵母和巴斯德毕赤酵母中表达核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）和磷酸核糖激酶（PRK）可以实现_CO2用于生产增值化学品的固定。此外，表达合成的CBB循环允许菌株在CO ₂上自养生长。在此，我们回顾了目前对CBB循环固定CO ₂的认识，总结了异养微生物中CBB循环组装或开发合成自养生物的研究进展，并提出了提高异养微生物CBB循环固碳效率的策略。还总结了此类应用在增强CO ₂固定以提高各种增值化学品生产方面的最新进展。进一步讨论了这一过程中遇到的挑战和未来的前景。

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Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO2 Fixation Based on Calvin–Benson–Bassham Cycle

Global climate change caused by greenhouse gas emission is the fundamental challenge facing mankind. Biological carbon dioxide fixation has recently attracted much attention. The Calvin–Benson–Bassham (CBB) cycle is a major carbon fixation pathway in the biosphere, and it is the most intensively studied because of its prominence and key role in nature as the primary pathway for net CO₂ fixation. It was recently shown that expressing the enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and phosphoribulokinase (PRK) in Escherichia coli, Saccharomyces cerevisiae, Methylobacterium extorquens, Kluyveromyces marxianus, and Pichia pastoris could achieve CO₂ fixation for the production of value-added chemicals. In addition, expressing the synthetic CBB cycle allowed strains to grow autotrophically on CO₂. Here, we review the current understanding of the CBB cycle for CO₂ fixation, summarize research progress in the assembly of the CBB cycle in heterotrophic microorganisms or developing synthetic autotrophs, and present the strategies to enhance carbon fixation efficiency of the CBB cycle in heterotrophic microorganisms. Recent progress in such applications in reinforcing CO₂ fixation for the improved production of various value-added chemicals is also summarized. The challenges encountered in this process and future prospects are further discussed.

更新日期：2023-06-21

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