Ultrasensitive Detection of SARS-CoV‑2 by Flexible Metal Oxide Field-Effect Transistors,Advanced Functional Materials

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Ultrasensitive Detection of SARS-CoV‑2 by Flexible Metal Oxide Field-Effect Transistors
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2023-06-19 , DOI: 10.1002/adfm.202301268
Sihui Hou _{1,

2} , Mengge Wu _{1,

2} , Hu Li ₂ , Hua‐Rui Gong ₃ , Zhan Gao ₂ , Rui Shi ₂ , Xingcan Huang ₂ , Dengfeng Li ₂ , Jian‐Dong Huang _{3,

4,

5,

6} , Junsheng Yu ₁ , Xinge Yu ₂

Affiliation

The pandemic of coronavirus disease 2019 (COVID-19) reflects the great significance of rapid and accurate detection of pathogens by new sensing technologies. Antibody based biosensors with high sensitivity comparable to golden standard polymerase chain reaction (PCR) and miniaturized device features allow the detection of pathogens in portable and flexible formats. Herein, flexible metal oxide electrolyte-gated field-effect transistors (EGFETs) are reported to serve as the biosensors for rapid and ultrasensitive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. The semiconducting layer of the EGFETs associates with hybrid material of PEI doped metal oxides that not only improves the transistor performance, but also regulates microstructure forming higher surface-to-volume ratio, which brings more antibodies immobilization, resulting in higher sensitive, and faster response for detecting SARS-CoV-2. Comprehensive studies of materials and interfacing engineering of the EGFETs not only build the strong foundation for the EGFET sensors to show excellent sensitivity with a limit of detection from 0.14 fg ml⁻¹ for SARS-CoV-2 S1 proteins, and 0.09 copies µl⁻¹ for SARS-CoV-2 viruses, but also offer good mechanical properties to enable thin, soft flexible sensing platforms. This work provides a new strategy from materials to devices as innovative schemes for virus/pathogens detection.

中文翻译：

通过柔性金属氧化物场效应晶体管对 SARS-CoV-2 进行超灵敏检测

2019冠状病毒病（COVID-19）的大流行体现了新传感技术快速、准确检测病原体的重要意义。基于抗体的生物传感器具有可与黄金标准聚合酶链式反应 (PCR) 相媲美的高灵敏度和小型化设备功能，允许以便携式和灵活的方式检测病原体。据报道，柔性金属氧化物电解质门控场效应晶体管 (EGFET) 可用作快速、超灵敏的严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 检测的生物传感器。EGFET的半导体层与PEI掺杂金属氧化物混合材料结合，不仅提高了晶体管性能，而且还调节微结构形成更高的表面积与体积比，从而带来更多的抗体固定化，从而提高检测 SARS-CoV-2 的灵敏度和更快的响应速度。对 EGFET 材料和接口工程的全面研究不仅为 EGFET 传感器奠定了坚实的基础，使其表现出出色的灵敏度，检测限可达 0.14 fg ml⁻¹对于 SARS-CoV-2 S1 蛋白，0.09 拷贝 µl ⁻¹对于 SARS-CoV-2 病毒，而且还提供良好的机械性能，以实现薄而柔软的柔性传感平台。这项工作提供了从材料到设备的新策略，作为病毒/病原体检测的创新方案。

更新日期：2023-06-19

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