斑马鱼（斑马鱼）幼虫轮胎磨损和道路颗粒渗滤液暴露不良后果的机制洞察,Environment International

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斑马鱼（斑马鱼）幼虫轮胎磨损和道路颗粒渗滤液暴露不良后果的机制洞察
Environment International ( IF 10.3 ) Pub Date : 2023-06-18 , DOI: 10.1016/j.envint.2023.108053
Jing Chang ₁ , Meng Jiao ₂ , Zhaoguang Zhang ₃ , Wentao Liu ₂ , Wei Li ₁ , Peng Xu ₁ , Bin Wan ₁

Affiliation

轮胎磨损颗粒（TWP）已成为我国主要的微塑料污染。含有 TWP 渗滤液的道路径流会减小水生生物的眼睛尺寸，甚至导致死亡。然而，TWP和道路颗粒（RP）渗滤液对水生生物的毒性机制仍不清楚。在这项研究中，斑马鱼胚胎暴露于环境相关浓度和高浓度的 TWP 或 RP 渗滤液中 5 天。从个体到分子水平筛选不良结果途径（AOP）。形态学和行为分析表明，渗滤液暴露主要损害斑马鱼幼虫的眼睛发育，抑制幼虫的游泳行为和趋光反应，产生不良后果。通过转录组学和代谢组学分析，斑马鱼视网膜调节的光转导显着下调。眼部组织病理学分析表明，渗滤液暴露后视网膜外核层（ONL）和视网膜色素上皮（RPE）厚度减少是由于感光细胞减少所致。此外，渗滤液暴露后，和基因的表达表现出浓度依赖性下调。光感受器细胞增殖的抑制被认为是斑马鱼幼虫眼中光感受器细胞减少的主要原因。该研究首次揭示了TWP和RP对斑马鱼幼虫眼睛的潜在毒性机制。

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Mechanistic insight into the adverse outcome of tire wear and road particle leachate exposure in zebrafish (Danio rerio) larvae

Tire wear particles (TWP) have become the major microplastic pollution in China. Road runoff containing TWP leachate can decrease the eye size and even induced mortality in the aquatic organisms. However, the toxic mechanism of TWP and road particles (RP) leachate on aquatic organisms is still unclear. In this study, the zebrafish embryos were exposed to TWP or RP leachate for 5 days at both environmental relevant and high concentrations. The adverse outcome pathways (AOPs) were screened from individual to molecular levels. The morphological and behavioral analysis demonstrated that the leachate exposure mainly impaired the eye development of zebrafish larvae and inhibited the larval swim behavior and phototactic response, which are the adverse outcomes. The phototransduction modulated by zebrafish retina was significantly down-regulated through transcriptomics and metabolomics analysis. The eye histopathological analysis showed that the decreased thickness of the retinal outer nuclear layer (ONL) and retinal pigmented epithelium (RPE) after leachate exposure were caused by the decreased photoreceptor cells. Moreover, the expression of and genes showed concentration-dependent down-regulation after leachate exposure. The inhibition of photoreceptor cell proliferation was identified as the main reason for photoreceptor cell decrease in zebrafish larval eye. This study, for the first time, uncovered the underlying toxic mechanism of TWP and RP on zebrafish larval eyes.

更新日期：2023-06-18

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