Abstract

Ethylene and gibberellin are two key phytohormones in regulating the postharvest physiological mechanism of plants. The present work aimed to understand the relationship between phytohormones and physiological responses to oxidative stress in transgenic (TR) and wild type (WT) roses at the bud and half-opening stages. The cut roses were subjected to exogenous exposure to 1 µL/L ethylene gas and 80 mg/L gibberellin (GA₃) at both stages for 24 h. The TR cut roses were less sensitive to ethylene as they contained the mutant etr1-1 gene. The main physiological parameters and endogenous phytohormones were measured in the outer ring of the petals by a factorial method in a completely randomized design in triplicates using GC, HPLC, and UV/VIS spectroscopy techniques. Endogenous ethylene and GA₃ increased and decreased with the flower opening and senescence in both lines, respectively. Antagonistic action in metabolism was observed by endogenous production and exogenous application of two phytohormones. GA₃ treatment significantly alleviated the postharvest-associated oxidative stress, so that the cut roses had the highest total protein, soluble carbohydrates, proline, and DPPH scavenging capacity, as well as the lowest MDA content and antioxidant enzyme activities at both bud and half-open stages. Both studied phytohormones can effectively control physiological responses associated with oxidative stress.

中文翻译：

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乙烯和赤霉素：刺激和减轻蔷薇采后相关氧化应激的两种关键植物激素。

摘要

乙烯和赤霉素是调节植物采后生理机制的两种关键植物激素。目前的工作旨在了解转基因（TR）和野生型（WT）玫瑰在花蕾和半开放阶段植物激素与氧化应激生理反应之间的关系。将切好的玫瑰在两个阶段均外源暴露于 1 µL/L 乙烯气体和 80 mg/L 赤霉素 (GA ₃ ) 中 24 小时。TR 切花玫瑰对乙烯不太敏感，因为它们含有突变体etr1-1基因。使用 GC、HPLC 和 UV/VIS 光谱技术，采用完全随机设计的阶乘方法，一式三份地测量花瓣外环的主要生理参数和内源植物激素。两个品系中内源乙烯和GA ₃分别随着花开放和衰老而增加和减少。通过两种植物激素的内源产生和外源应用观察到代谢中的拮抗作用。遗传算法₃处理显着缓解了采后相关的氧化应激，使得切花玫瑰花蕾期和半开期的总蛋白、可溶性碳水化合物、脯氨酸和DPPH清除能力最高，MDA含量和抗氧化酶活性最低。两种研究的植物激素都可以有效控制与氧化应激相关的生理反应。