ABSTRACT

Glyphosate herbicide treatment is essential to sustainable Eucalyptus plantation management in Brazil. Eucalyptus is highly sensitive to glyphosate, and Suzano/FuturaGene has genetically modified eucalyptus to tolerate glyphosate, with the aim of both protecting eucalyptus trees from glyphosate application damage and improving weed management. This study presents the biosafety results of the glyphosate-tolerant eucalyptus event 751K032, which expresses the selection marker neomycin phosphotransferase II (NPTII) enzyme and CP4-EPSPS, a glyphosate-tolerant variant of plant 5-enolpyruvyl-shikimate−3-phosphate synthase enzyme. The transgenic genetically modified (GM) event 751K032 behaved in the plantations like conventional non-transgenic eucalyptus clone, FGN-K, and had no effects on arthropods and soil microorganisms. The engineered NPTII and CP4 EPSPS proteins were heat-labile, readily digestible, and according to the bioinformatics analyses, unlikely to cause an allergenic or toxic reaction in humans or animals. This assessment of the biosafety of the glyphosate-tolerant eucalyptus event 751K032 concludes that it is safe to be used for wood production.

摘要

草甘膦除草剂处理对于巴西桉树种植园的可持续管理至关重要。桉树对草甘膦高度敏感，Suzano/FuturaGene 对桉树进行了基因改造，使其能够耐受草甘膦，目的是保护桉树免受草甘膦施用损害并改善杂草管理。本研究介绍了耐草甘膦桉树事件 751K032 的生物安全性结果，该事件表达选择标记新霉素磷酸转移酶 II (NPTII) 酶和 CP4-EPSPS（植物 5-烯醇丙酮酰莽草酸−3-磷酸合酶的耐草甘膦变体） 。转基因转基因（GM）事件751K032在种植园中的表现与传统的非转基因桉树克隆FGN-K类似，并且对节肢动物和土壤微生物没有影响。工程化的 NPTII 和 CP4 EPSPS 蛋白不耐热、易于消化，并且根据生物信息学分析，不太可能在人类或动物中引起过敏或毒性反应。对耐草甘膦桉树事件 751K032 的生物安全性评估得出的结论是，将其用于木材生产是安全的。