Abstract—

In this work, we report on the effect of Shewanella oneidensis MR-1 and Bacillus subtilis 168 bacteria used for the biosynthesis of CdS nanoparticles (CdS NPs) on the level of antimicrobial activity of CdS NPs/Shewanella and CdS NPs/Bacillus against a wide range of microorganisms: gram-positive, gram-negative bacteria, yeast and mold fungi. It is shown that S. oneidensis MR-1 and B. subtilis determine the quantitative characteristics of the nanomaterial, the content of protein and the CdS fraction in CdS NP samples. The use of the atomic emission spectroscopy method to determine the elemental content of cadmium and sulfur, as well as the densitometric analysis of electropherograms of protein adsorbed on the surface of biogenic CdS NPs demonstrate a significant difference in the ratio of these components in CdS NPs/Shewanella and CdS NPs/Bacillus. It is found that the proportion of CdS in CdS NPs/Bacillus exceeds by 1.4 times this parameter for CdS NPs/Shewanella. At the same time, the proportion of protein in CdS NPs/Bacillus is reduced compared to CdS NPs/Shewanella. It is shown that the level of antimicrobial activity of CdS NPs/Bacillus in relation to all test cultures studied exceeds the same parameter for CdS NPs/Shewanella. It is assumed that an increase in the proportion of CdS in CdS NPs/Bacillus may be the cause of a higher level of biocidal activity of CdS NPs due to an increased amount of released Cd²⁺ ions, which lead to the generation of reactive oxygen species and the destruction of microbial cells. Thus, the importance and necessity of selecting bacterial strains for the creation of functionally active nanoparticles with optimal characteristics is proved. The results of this research confirm the prospects of utilizing biogenic CdS NPs as targeted antimicrobial agents.

中文翻译：

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生产硫化镉纳米颗粒的菌株对纳米材料杀菌活性水平的影响

摘要——

在这项工作中，我们报告了用于生物合成 CdS 纳米颗粒 (CdS NPs) 的Shewanella oneidensis MR-1 和Bacillus subtilis 168 细菌对CdS NPs/ Shewanella和 CdS NPs/ Bacillus的抗菌活性水平的影响。微生物范围：革兰氏阳性菌、革兰氏阴性菌、酵母菌和霉菌。结果表明，S. oneidensis MR-1 和B. subtilis确定纳米材料的定量特征、CdS NP 样品中蛋白质的含量和 CdS 分数。利用原子发射光谱法测定镉和硫元素含量，以及对生物CdS NPs表面吸附的蛋白质电泳图进行光密度分析，表明CdS NPs/中这些成分的比例存在显着差异。希瓦氏菌和 CdS NP/芽孢杆菌。发现CdS NPs/芽孢杆菌中CdS的比例超过CdS NPs/希瓦氏菌的该参数的1.4倍。同时，与CdS NPs/希瓦氏菌相比，CdS NPs/芽孢杆菌中的蛋白质比例有所降低。结果表明，与所研究的所有测试培养物相关的CdS NP/芽孢杆菌的抗菌活性水平超过了 CdS NP/希瓦氏菌的相同参数。据推测，CdS NP/芽孢杆菌中 CdS 比例的增加可能是由于释放的 Cd ²⁺离子数量增加而导致 CdS NP 的杀菌活性水平更高，从而导致活性氧的产生物种和微生物细胞的破坏。因此，证明了选择菌株来创建具有最佳特性的功能活性纳米颗粒的重要性和必要性。这项研究的结果证实了利用生物 CdS NPs 作为靶向抗菌剂的前景。